Strains or plasmids | Descriptions | Reference or source |
---|---|---|
K. pneumoniae | Â | Â |
CG43S3 | CG43 Smr | [56] |
ΔlacZ | CG43S3ΔlacZ | [17] |
Δfur | CG43S3Δfur | [22] |
ΔlacZ Δfur | CG43S3ΔlacZ Δfur | [22] |
ΔryhB | CG43S3ΔryhB | This study |
Δfur ΔryhB | CG43S3Δfur ΔryhB | This study |
ΔlacZ Δfur ΔryhB | CG43S3ΔlacZ Δfur ΔryhB | This study |
ΔgalU | CG43S3ΔgalU | [57] |
E. coli | Â | Â |
DH5α | supE44 ΔlacU169 (f80 lacZ Δμ15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [58] |
BL21-RIL | F-ompT hsdS B [r B -m B -]gal dcm [DE3] | Laboratory stock |
S17-1 λ pir H1717 | hsdR recA pro RP4-2 [Tc::Mu; Km::Tn7] [λpir] araD139 ΔlacU169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR aroB fhuF::λ placMu | |
Plasmids | Â | Â |
pKAS46 | Positive selection suicide vector, rpsL Apr Kmr | [59] |
yT&A | TA cloning vector | Yeastern |
pRK415 | Broad-host-range IncP cloning vector, Tcr | [61] |
pT7-7 | Cloning vector, Apr | [62] |
pETQ | Kmr, protein expression vector | [61] |
placZ15 | Cmr, promoter selection vector, lacZ+ | [17] |
pfur | Tcr, 0.8-kb fragment containing a fur allele cloned into pRK415 | [22] |
pET30c-Fur | Kmr, 450-bp fragment encoding full-length Fur cloned into pET30c | [22] |
pRyhB04 | 2.0 kb fragment containing an internal ~70-bp deletion in ryhB cloned into pKAS46 | This study |
pRyhB15 | Cmr, 178-bp fragment containing the region upstream of ryhB cloned into placZ15 | This study |
pOrf12 | Cmr, 500-bp fragment containing the region upstream of Klebsiella K2 cps orf1-orf2 cloned into placZ15 | [17] |
pOrf315 | Cmr, 900-bp fragment containing the region upstream of Klebsiella K2 cps orf3-orf15 cloned into placZ15 | [17] |
pOrf1617 | Cmr, 300-bp fragment containing the region upstream of Klebsiella K2 cps orf16-orf17 cloned into placZ15 | [17] |
pT7-7-pryhB | 178-bp fragment containing the putative ryhB promoter, cloned into pT7-7 | This study |
pETQ-ryhB | Kmr, 326-bp fragment containing the promoter and coding region of ryhB cloned into pETQ | This study |