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Table 2 Bacterial strains and plasmids used in this study

From: Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model

Strain or plasmid Description Reference or source
Strains   
E058 APEC O2 (Ent+ Sal+ Aer+ Ybt+) [49]
U17 UPEC nontypable (Ent+ Sal+ Aer+Ybt+), Nalr Tcr [5]
E058Δ chuT Δ chuT :: kan, Kanr This study
E058Δ iroD Δ iroD :: cam, Camr This study
E058Δ iucD Δ iucD :: zeo, Zeor This study
E058Δ chuT Δ iroD Δ iucD Δ chuT :: kan Δ iroD :: cam Δ iucD :: zeo, Kanr Camr Zeor This study
U17Δ chuT Δ chuT :: kan, Kanr This study
U17Δ iroD Δ iroD :: cam, Camr This study
U17Δ iucD Δ iucD :: zeo, Zeor This study
U17Δ chuT Δ iroD Δ iucD Δ chuT :: kan Δ iroD :: cam Δ iucD :: zeo, Kanr Camr Zeor This study
ReE058TripiroD pGEX-6p-1-iroD complementation to E058Δ chuT Δ iroD Δ iucD, This study
  Ampr  
ReU17TripiroD pGEX-6p-1-iroD complementation to U17Δ chuT Δ iroD Δ iucD, This study
  Ampr  
Plasmid   
pMD18-T Simple Vector TA Cloning Vector Takara
pMD-chuT chuT cloned into pMD 18-T Simple Vector This study
pMD-iroD iroD cloned into pMD 18-T Simple Vector This study
pMD-iucD iucD cloned into pMD 18-T Simple Vector This study
pEM7/Zeo Zeocin-resistant cassette Invitrogen
pUC4K Kanamycin-resistant cassette Invitrogen
pKD3 λ red template vector; Camr Ampr [50]
pKD46 Red recombinase helper plasmid, temp sensitive; Ampr [50]
pMD-chuT-Kan Kan -resistant gene inserted into pMD-chuT This study
pMD-iroD-Cam Cam-resistant gene inserted into pMD-iroD This study
pMD-iucD-Zeo Zeo-resistant gene inserted into pMD-iucD This study
pGEX-6p-1 expression vector Amersham
pGEX-6p-1-iroD Bam HI-Eco RIiroD fragment cloned into pGEX-6p-1 This study
  1. aEnt, enterobactin; Sal, salmochelin; Aer, aerobactin; Ybt, yersiniabactin; Kan, kanamycin; Cam, chloramphenicol; Amp, ampicillin; Zeo, zeocin; Nal, nalidixic acid; Tc, tetracycline.