Table 2 Bacterial strains and plasmids used in this study
Strain or plasmid | Description | Reference or source |
|---|---|---|
Strains | Â | Â |
E058 | APEC O2 (Ent+ Sal+ Aer+ Ybt+) | [49] |
U17 | UPEC nontypable (Ent+ Sal+ Aer+Ybt+), Nalr Tcr | [5] |
E058Δ chuT | Δ chuT :: kan, Kanr | This study |
E058Δ iroD | Δ iroD :: cam, Camr | This study |
E058Δ iucD | Δ iucD :: zeo, Zeor | This study |
E058Δ chuT Δ iroD Δ iucD | Δ chuT :: kan Δ iroD :: cam Δ iucD :: zeo, Kanr Camr Zeor | This study |
U17Δ chuT | Δ chuT :: kan, Kanr | This study |
U17Δ iroD | Δ iroD :: cam, Camr | This study |
U17Δ iucD | Δ iucD :: zeo, Zeor | This study |
U17Δ chuT Δ iroD Δ iucD | Δ chuT :: kan Δ iroD :: cam Δ iucD :: zeo, Kanr Camr Zeor | This study |
ReE058TripiroD | pGEX-6p-1-iroD complementation to E058Δ chuT Δ iroD Δ iucD, | This study |
| Â | Ampr | Â |
ReU17TripiroD | pGEX-6p-1-iroD complementation to U17Δ chuT Δ iroD Δ iucD, | This study |
| Â | Ampr | Â |
Plasmid | Â | Â |
pMD18-T Simple Vector | TA Cloning Vector | Takara |
pMD-chuT | chuT cloned into pMD 18-T Simple Vector | This study |
pMD-iroD | iroD cloned into pMD 18-T Simple Vector | This study |
pMD-iucD | iucD cloned into pMD 18-T Simple Vector | This study |
pEM7/Zeo | Zeocin-resistant cassette | Invitrogen |
pUC4K | Kanamycin-resistant cassette | Invitrogen |
pKD3 | λ red template vector; Camr Ampr | [50] |
pKD46 | Red recombinase helper plasmid, temp sensitive; Ampr | [50] |
pMD-chuT-Kan | Kan -resistant gene inserted into pMD-chuT | This study |
pMD-iroD-Cam | Cam-resistant gene inserted into pMD-iroD | This study |
pMD-iucD-Zeo | Zeo-resistant gene inserted into pMD-iucD | This study |
pGEX-6p-1 | expression vector | Amersham |
pGEX-6p-1-iroD | Bam HI-Eco RIiroD fragment cloned into pGEX-6p-1 | This study |