Skip to main content

Table 2  rrs  gene based primers used for qPCR quantification and PCR-DGGE

From: Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep

Target organism

Primer set

Primer sequences 5' - 3'

Assay

Reference

Total bacteria

520 F

AGCAGCCGCGGTAAT

qPCR

[14]

 

799 R2

CAGGGTATCTAATCCTGTT

  

Fibrobacter

FibSuc3F

GCGGGTAGCAAACAGGAT TAGA

qPCR

[15]

succinogenes

FibSuc3R

CCCCCGGACACCCAGTAT

  

Ruminococcus

RumAlb3F

TGTTAACAGAGGGAAGCAAAGCA

qPCR

[15]

albus

RumAlb3R

TGCAGCCTACAATCCGAACTAA

  

Ruminococcus

RumFla3F

TGGCGGACGGGTGAGTAA

qPCR

[15]

flavefaciens

RumFla3R

TTACCATCCGTTTCCAGAAGC T

  

Genus

PrevGen4F

GGTTCTGAGAGGAAGGTCCCC

qPCR

[15]

Prevotella

PrevGen4R

TCCTGCACGCTACTTGGCTG

  

Streptococcus

StrBov2F

TTCCTAGAGATAGGAAGTTTCTTC GG

qPCR

[15]

bovis

StrBov2R

ATG ATG GCA ACT AAC AAT AGG GGT

  

Genus

Lacto 05 F

AGC AGT AGG GAA TCT TCC A

qPCR

[16]

Lactobacillus

Lacto 04R

CGCCACTGGTGTTCYTCCATATA

  

Total bacteria

GC + Eub340F

CGCCCGCCGCGCGCGGCGGGCGGGGCG GGGGCACGGGGGG TCCTACGGGAGGCAGCAG

DGGE

[1719]

 

HDA2R

GTA TTA CCG CGG CTG CTG GCA C