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Table 7 FISH probes targeting 16S rRNA and the hybridization conditions used in this study

From: Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

Probe

Target

Target sequence

E.coli positions

Formamide (%)

NaCl (mM)

Fluorophore

Reference

ARC915

Archaea

GTGCTCCCCCGCCAATTCCT

915-934

35

70

Cy5

[73],[74]

MX825

Methanosaetaceae

TCGCACCGTGGCCGACACCTAGC

825-845

50

18

Cy3

[73],[74]

EUB338

Bacteria

GCTGCCTCCCGTAGGAGT

338-355

35

70

Alexa

[75]

EUB338 II

Bacteria

GCAGCCACCCGTAGGTGT

338-355

35

70

Alexa

[75]

EUB338 III

Bacteria

GCTGCCACCCGTAGGTGT

338-355

35

70

Alexa

[75]