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Table 3 Observed and predicted TRF lengths a

From: Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

Observed TRF lengths T-RFLP 2007-05-22

Predicted TRF lengths, clone library 2007-05-22

AluI

%b

RsaI

%b

AluI

RsaI

%c

Identity

    

84

80

1%

Thaumarchaeota/Cluster 1.3

    

126

608

1%

Thermoplasmatales/Cluster B

    

166

609

2%

Thermoplasmatales/Cluster B

    

180

80

4%

Methanosaeta

183

100%

74

86%

188

80

48%

Methanosaeta

  

238

14%

188

242

21%

Methanosaeta

    

188

NDd

2%

Methanosaeta

    

189

264

1%

ARC I

    

359

262

1%

Thaumarchaeota/Cluster 1.1b

    

400

609

1%

Thermoplasmatales/RCIII

    

515

264

1%

Methanocorpusculum

    

551

609

10%

Thermoplasmatales/RCIII

    

ND

80

1%

Methanosaeta

    

ND

613

1%

Thermoplasmatales/Cluster C

    

ND

ND

4%

Methanosaeta

  1. a Observed and predicted TRF lengths from T-RFLP and clone library analysis of a sample from 2007-05-22. b Relative abundance based on total fluorescence. c Relative abundance based on frequency in clone library. d ND indicates cases where a TRF could not be predicted or where the predicted TRF was outside the detection range.