Strain or plasmid | Relevant characteristic | Reference or source |
---|---|---|
Strains | Â | Â |
R. rubrum | Â | Â |
S1 | Wild type | Â |
E. coli | Â | Â |
BL21 (DE3) pLysS | Host for expression of PII proteins, Cmr | Invitrogen |
BL21 Star (DE3) | Host for expression of GlnE | Invitrogen |
RB9040 | ΔglnD; host for expression of GlnD, Tcr | [19] |
Plasmids | Â | Â |
pETGlnE | pET101 derivative containing glnE, Apr | [5] |
pGEXGlnD | pGEX6P-3 derivative containing glnD, Apr | [11] |
pMJET | pET15b derivative containing glnB, Apr | [20] |
pETGlnJ | pET15b derivative containing glnJ, Apr | [5] |
pETGlnJR17K | pETGlnJ derivative encoding GlnJR17K, Apr | This study |
pETGlnJQ42H | pETGlnJ derivative encoding GlnJQ42H, Apr | This study |
pETGlnJN54D | pETGlnJ derivative encoding GlnJN54D, Apr | This study |
pETGlnJK85R | pETGlnJ derivative encoding GlnJK85R, Apr | This study |
pETGlnJV100I | pETGlnJ derivative encoding GlnJV100I, Apr | This study |
pETGlnJE109G | pETGlnJ derivative encoding GlnJE109G, Apr | This study |
pETGlnJQ42HK85R | pETGlnJ derivative encoding GlnJQ42HK85R, Apr | This study |
pETGlnBH42Q | pMJET derivative encoding GlnBH42Q, Apr | This study |
pETGlnBR85K | pMJET derivative encoding GlnBR85K, Apr | This study |
pETGlnBH42QR85K | pMJET derivative encoding GlnBH42QR85K, Apr | This study |