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Table 1 Bacterial strains and plasmids used in the present study

From: Molecular basis for the distinct divalent cation requirement in the uridylylation of the signal transduction proteins GlnJ and GlnB from Rhodospirillum rubrum

Strain or plasmid

Relevant characteristic

Reference or source

Strains

  

R. rubrum

  

S1

Wild type

 

E. coli

  

BL21 (DE3) pLysS

Host for expression of PII proteins, Cmr

Invitrogen

BL21 Star (DE3)

Host for expression of GlnE

Invitrogen

RB9040

ΔglnD; host for expression of GlnD, Tcr

[19]

Plasmids

  

pETGlnE

pET101 derivative containing glnE, Apr

[5]

pGEXGlnD

pGEX6P-3 derivative containing glnD, Apr

[11]

pMJET

pET15b derivative containing glnB, Apr

[20]

pETGlnJ

pET15b derivative containing glnJ, Apr

[5]

pETGlnJR17K

pETGlnJ derivative encoding GlnJR17K, Apr

This study

pETGlnJQ42H

pETGlnJ derivative encoding GlnJQ42H, Apr

This study

pETGlnJN54D

pETGlnJ derivative encoding GlnJN54D, Apr

This study

pETGlnJK85R

pETGlnJ derivative encoding GlnJK85R, Apr

This study

pETGlnJV100I

pETGlnJ derivative encoding GlnJV100I, Apr

This study

pETGlnJE109G

pETGlnJ derivative encoding GlnJE109G, Apr

This study

pETGlnJQ42HK85R

pETGlnJ derivative encoding GlnJQ42HK85R, Apr

This study

pETGlnBH42Q

pMJET derivative encoding GlnBH42Q, Apr

This study

pETGlnBR85K

pMJET derivative encoding GlnBR85K, Apr

This study

pETGlnBH42QR85K

pMJET derivative encoding GlnBH42QR85K, Apr

This study

  1. Ap ampicillin; Tc tetracycline; Cm chloramphenicol.