Figure 2From: Molecular basis for the distinct divalent cation requirement in the uridylylation of the signal transduction proteins GlnJ and GlnB from Rhodospirillum rubrumUridylylation of GlnJ (A) and GlnB (B) variants. The reactions were performed as described in the Materials and methods in the presence of Mn2+, Mg2+ or without either divalent cation (control - C), and the uridylylation status analyzed by native PAGE. U – unmodified, M3- modified (fully modified trimmers).Back to article page