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Table 2 Redox potentials of the assay buffers

From: Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of Escherichia coli K-12

Hydrogen in headspace

50 mM MOPS, pH 7

50 mM MOPS, pH 7, BV/TTCa

50 mM MOPS, pH 7, PMS/NBTb

50 mM MOPS, pH 7, NBT

0%c

+ 170 mV

+ 78 mV

+ 74 mV

+ 73 mV

5%

- 120 mV

- 264 mV

- 38 mV

- 65 mV

100%

- 349 mV

- 322 mV

- 92 mV

- 102 mV

  1. a The concentrations of BV and TTC were 0.5 mM and 1.0 mM, respectively.
  2. b The concentrations of PMS and NBT were 0.3 mM and 0.2 mM, respectively.
  3. c Measured at 25 °C and 1 atm. pressure. 0% hydrogen indicates measurements were made in air. Note that all measurements were made twice.