Table 3 Summarised Purification steps of ACP
From: Biochemical characterization of an anti-Candida factor produced by Enterococcus faecalis
Purification stage | Volume (mL) | Activity (AU mL-1) | Protein (mg mL-1) | Specific activity (AUmg-1protein) | Purification factor | Recovery (%) |
|---|---|---|---|---|---|---|
Culture Supernatant | 400 | 1600 | 0.4025 | 39751 | 1 | 100 |
Ammonium sulfate and dialysis | 10 | 3200 | 0.0444 | 72072 | 1.8 | 11 |
Ion Exchange Chromatography | 6 | 1600 | 0.0023 | 695652 | 17.5 | 0.57 |
Gel Filtration | 2 | 1600 | 0.0018 | 888888 | 22.4 | 0.45 |