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Table 1 Mean diameter (± standard deviation)* and evidence of pigment secretion of S. nodorum strains growing in the dark on minimal medium supplemented with different carbon sources

From: A comparative analysis of the heterotrimeric G-protein Gα, Gβ and Gγ subunits in the wheat pathogen Stagonospora nodorum

Media supplement

S. nodorum strain

Diameter1(mm)

Colour of secretion

Arabinose

SN15

61.5 ± 2.4 (A)

NA

 

gna 1

36.2 ± 1.0 (CD)

NA

 

gba 1

45.5 ± 0.6 (BC)

yellow

 

gga A

25.5 ± 1.0 (E)

NA

Fructose

SN15

59.2 ± 1.3 (A)

NA

 

gna 1

45 ± 0.8 (BC)

NA

 

gba 1

43.7 ± 2.1 (BC)

NA

 

gga A

31.7 ± 2.1 (D)

NA

Glucose

SN15

61.7 ± 1.5 (A)

NA

 

gna 1

39.5 ± 0.6 (C)

deep orange

 

gba 1

48.7 ± 1.7 (B)

dark brown

 

gga A

37.5 ± 0.6 (C)

light brown

Lactose

SN15

52.5 ± 1.3 (B)

NA

 

gna 1

36.7 ± 1.0 (CD)

NA

 

gba 1

40.2 ± 0.5 (C)

yellow

 

gga A

31.0 ± 1.2 (D)

NA

Mannitol

SN15

51.0 ± 1.8 (B)

NA

 

gna 1

38.0 ± 0 (C)

NA

 

gba 1

39.2 ± 1.0 (C)

yellow

 

gga A

28.7 ± 1.0 (D)

NA

Media supplement

S. nodorum strain

Diameter (mm)

Colour of secretion

Sucrose

SN15

60.2 ± 3.9 (A)

NA

 

gna 1

42.2 ± 0.5 (C)

NA

 

gba 1

50.5 ± 2.4 (B)

NA

 

gga A

33.2 ± 1.0 (D)

NA

Trehalose

SN15

60 ± 0.8 (A)

NA

 

gna 1

35.5 ± 0.6 (CD)

NA

 

gba 1

39.7 ± 1.5 (C)

NA

 

gga A

36.2 ± 2.2 (CD)

NA

Glucose + NaCl

SN15

33.2 ± 0.5 (D)

NA

 

gna 1

18.5 ± 0.6 (E)

dark brown

 

gba 1

21.2 ± 0.5 (E)

NA

 

gga A

22.0 ± 1.2 (E)

NA

Casamino - NaNO3

SN15

50.7 ± 1.3 (B)

NA

 

gna 1

35.2 ± 1.3 (CD)

NA

 

gba 1

35.2 ± 1.9 (CD)

NA

 

gga A

33.2 ± 0.5 (D)

NA

Glucose + Casamino

SN15

52.0 ± 1.2 (B)

orange/brown

 

gna 1

37.7 ± 0.5 (CD)

orange/brown

 

gba 1

43.0 ± 0.8 (BC)

orange/brown

 

gga A

40.7 ± 1.0 (C)

orange/brown

  1. Wild-type SN15 and the mutant strains gna1-35, gba1-6 and ggaA-25 display carbon source-dependent changes in radial growth rate and pigment secretion as observed 10 days post inoculation (dpi).
  2. 1The letters in brackets shown after the diameter measurements define the significance of the results with growth assays showing the same letter being not significantly different (p < 0.05).