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Figure 3 | BMC Microbiology

Figure 3

From: Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767

Figure 3

pH and temperature dependence of recombinant Pc Aad1p activity. (A) Effect of pH in reduction and oxidation reactions. Reduction activities were measured at pH 5.5-8.8 with 0.2 mM NADPH and 0.2 mM 3,4-Dimethoxybenzaldehyde. Oxidation reactions were performed at pH 9.0-10.7 using 0.3 mM NADP + and 10 mM 3,4-Dimethoxybenzyl alcohol. Reactions were carried out at 30°C. (B) Effect of temperature on the reduction activity of recombinant Pc Aad1p. Activity was measured at pH 6.1 with NADPH and 3,4-Dimethoxybenzaldehyde at 0.2 mM final concentration. The reaction was started by adding 9.0 μg of the enzyme. Results are the mean ± SEM from two separate experiments.

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