Figure 5
From: Bacillus cereus Fnr binds a [4Fe-4S] cluster and forms a ternary complex with ResD and PlcR
Far-Western analysis of PlcR-Fnr, PlcR-ResD and ResD-Fnr interactions. Increased amounts of purified Fnr, ResD and PlcR were spotted onto nitrocellulose membranes and incubated with biotinylated-PlcR (A) or biotinylated-ResD (B), under anoxic conditions. PlcR and ResD binding was detected using streptavidin-HRP complex and visualized by chemiluminescence. BSA was used as negative control.