Figure 5

Effect of different mechanism based inhibitors (A) PMSF, (B) LY2183240 and (C) MAFP on Dictyostelium FAAH activity. 10μg of HIS-FAAH protein purified from Dictyostelium were incubated for 30 min at 37°C with 100μM arachidonoyl p- nitroaniline substrate in the absence (0 mM) or presence of increasing concentration of PMSF, LY2183240 and MAFP. Calculated specific activity of the enzyme reactions with and without the inhibitors were represented as % relative activity. The data are means ± S.D. of three replicate experiments.