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Table 1 Plasmids and oligonucleotide primers
| Plasmid | Characteristics | Source or Reference |
|---|---|---|
| pCR2.1-TOPO | Cloning vector, kanamycin resistance and ampicillin resistance genes | Invitrogen |
| pCP0 | Vector for gene expression in mycobacteria, kanamycin resistance gene | [4] |
| pCP0-gplH | pCP0 expressing M. smegmatis gplH | This study |
| pCP0-mbtHMs | pCP0 expressing M. smegmatis mbtH (MSMEG_4508) | [35] |
| p2NIL | Kanamycin resistance gene and OriE | [57] |
| pGOAL19 | Hygromycin resistance gene, sacB-lacZ PacI cassette, and OriE | [57] |
| p2NIL-GOALc-ΔgplHc | Delivery vector carrying a gplH deletion cassette (ΔgplHc) | This study |
| Oligonucleotide | Sequence (5’ to 3’) | Characteristics |
| pepOF | GGTACCTGTTCAACGCGGCCAGAGCGTCATTGGTCTCGGCCA | KpnI |
| pepOR | TTAATTAATGTTGCAACAGCTCCCTGATCCGGATGTCGACGTGCTTG | PacI |
| pepIR | TCAGCCGTCAAGAGCAAAGCTGCCGTTGTCGTCATCGAACGGGTTGAT | SOE PCR |
| pepIF | CGACAACGGCAGCTTTGCTCTTGACGGCTGAGTCAAATAGTCTGTTG | SOE PCR |
| pepF | CTGCAGTGAACAGCCGGGAGAAACGT | PstI |
| pepR | AAGCTTCCCAACAGACTATTTGACTCAGCCG | HindIII |
