|
pCR2.1-TOPO
|
Cloning vector, kanamycin resistance and ampicillin resistance genes
|
Invitrogen
|
|
pCP0
|
Vector for gene expression in mycobacteria, kanamycin resistance gene
|
[4]
|
|
pCP0-gplH
|
pCP0 expressing M. smegmatis gplH
|
This study
|
|
pCP0-mbtHMs
|
pCP0 expressing M. smegmatis mbtH (MSMEG_4508)
|
[35]
|
|
p2NIL
|
Kanamycin resistance gene and OriE
|
[57]
|
|
pGOAL19
|
Hygromycin resistance gene, sacB-lacZ PacI cassette, and OriE
|
[57]
|
|
p2NIL-GOALc-ΔgplHc
|
Delivery vector carrying a gplH deletion cassette (ΔgplHc)
|
This study
|
|
Oligonucleotide
|
Sequence (5’ to 3’)
|
Characteristics
|
|
pepOF
|
GGTACCTGTTCAACGCGGCCAGAGCGTCATTGGTCTCGGCCA
|
KpnI
|
|
pepOR
|
TTAATTAATGTTGCAACAGCTCCCTGATCCGGATGTCGACGTGCTTG
|
PacI
|
|
pepIR
|
TCAGCCGTCAAGAGCAAAGCTGCCGTTGTCGTCATCGAACGGGTTGAT
|
SOE PCR
|
|
pepIF
|
CGACAACGGCAGCTTTGCTCTTGACGGCTGAGTCAAATAGTCTGTTG
|
SOE PCR
|
|
pepF
|
CTGCAGTGAACAGCCGGGAGAAACGT
|
PstI
|
|
pepR
|
AAGCTTCCCAACAGACTATTTGACTCAGCCG
|
HindIII
|
