Name | Description a | Reference or source |
---|---|---|
Salmonella enterica serotype Typhimurium | ||
LB5010 | Wild type S. enterica serotype Typhimurium LT2 strain, fimbriate with the complete fim gene cluster | [21] |
Δstm0551 | stm0551 deletion mutant; Kanr | Present study |
Escherichia coli strain | ||
One Shot® TOP10 chemically competent E. coli | F-mcr A Δ(mrr-hsd RMS-mcr BC) Φ80lacZ ΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (StrR) end A1 nup G | Invitrogen |
BL21Star™ (DE3) One Shot® chemically competent E. coli | F-ompT hsdS B (rB- mB-) gal dcm (DE3) | Invitrogen |
Plasmids | ||
pSTM0551 | A 0.5-kb DNA fragment possessing the stm0551 coding sequence cloned into the pACYC184 vector; Cmr | Present study |
pSTM0551E49A | A 0.5-kb DNA fragment possessing the stm0551 coding sequence with glutamic acid (E) at position 49 replaced with an alanine (A) cloned into the pACYC184 vector; Cmr | Present study |
pACYC184 | Tetr, Cmr, cloning vector; w/p15A ori | ATCC, Manassas, VA |
pET101/D-TOPO | Ampr, 6xHis tag expression vector | Invitrogen |
pKD46 | Ampr, express λ Red recombinase system, temp- sensitive replicon | [22] |
pKD13 | Plasmid carrying Kanr cassette | [22] |