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Table 1 Bacterial strains and plasmids used in this study

From: A previously uncharacterized gene stm0551 plays a repressive role in the regulation of type 1 fimbriae in Salmonella enterica serotype Typhimurium

Name

Description a

Reference or source

Salmonella enterica serotype Typhimurium

LB5010

Wild type S. enterica serotype Typhimurium LT2 strain, fimbriate with the complete fim gene cluster

[21]

Δstm0551

stm0551 deletion mutant; Kanr

Present study

Escherichia coli strain

One Shot® TOP10 chemically competent E. coli

F-mcr A Δ(mrr-hsd RMS-mcr BC) Φ80lacZ ΔM15 Δlac X74 rec A1 ara D139Δ(ara-leu)7697 gal U gal K rps L (StrR) end A1 nup G

Invitrogen

BL21Star™ (DE3) One Shot® chemically competent E. coli

F-ompT hsdS B (rB- mB-) gal dcm (DE3)

Invitrogen

Plasmids

pSTM0551

A 0.5-kb DNA fragment possessing the stm0551 coding sequence cloned into the pACYC184 vector; Cmr

Present study

pSTM0551E49A

A 0.5-kb DNA fragment possessing the stm0551 coding sequence with glutamic acid (E) at position 49 replaced with an alanine (A) cloned into the pACYC184 vector; Cmr

Present study

pACYC184

Tetr, Cmr, cloning vector; w/p15A ori

ATCC, Manassas, VA

pET101/D-TOPO

Ampr, 6xHis tag expression vector

Invitrogen

pKD46

Ampr, express λ Red recombinase system, temp- sensitive replicon

[22]

pKD13

Plasmid carrying Kanr cassette

[22]

  1. aAmpr ampicillin resistant; Cmr chloramphenicol resistant; Kanr kanamycin resistant; Tetr tetracycline resistant