Table 1 Bacterial strains and plasmids

E. coli

TG2

supE hsdΔ5 thiΔ (lac-proAB) F' Δ(srl-recA)306::Tn10(Tc^r) lacZΔM15

[31]

BL21(DE3)pLysS

F^-ompT hsdS _B (r_B- m_B-) gal dcm (DE3) pLysS (Cm^r)

Novagen

C. metallidurans

CH34

Zn, Cd, Co, Pb, Cu, Hg, Ni and Cr resistance

[6]

AE104

Plasmid-cured C. metallidurans strain- sensitive to toxic metals

[6]

Plasmid

Description

Reference or source

pET32LIC

Ap^r Overexpression plasmid for ligation-independent cloning

Novagen

pET32LIC pbrR

Ap^rpbrR cloned into pET32LIC

This study

pMa5/8

Ap^r Cm^s Mutagenesis vector

[32]

pMc5/8

Ap^s Cm^r Mutagenesis vector

[32]

pMaPbrR/PpbrA

Ap^r Cm^s Mutagenesis vector with pbrR/PpbrA cloned in to it

This study

pMOL1139

Km^r, The pbr operon cloned into plasmid pRK415

B. Borremans

pMU2385

Tp^r 13.3 kb low copy number lacZ reporter plasmid

[33]

pMUPpbrA

Tp^r pMU2385 containing the PpbrA promoter directing lacZ transcription

This study

pMUPpbrA-1

Tp^r pMU2385 containing the PpbrA promoter with a 1 bp deletion

This study

pMUPpbrAcon

Tp^r As pMUPpbrA, but −10 sequence changed to E. coli consensus

This study

pMUPpbrAmer

Tp^r As pMUPpbrA, but −10 sequence changed to mer promoter

This study

pMUPbrR/PpbrA

Tp^r, pMU2385 containing pbrR, PpbrA ΔpbrA directing lacZ transcription

This study

pMUPbrRC14S/PpbrA

As pMUPbrRPpbrA, but PbrR C14S

This study

pMUPbrRC55S/PpbrA

As pMUPbrRPpbrA, but PbrR C55S

This study

pMUPbrRC79S/PpbrA

As pMUPbrRPpbrA, but PbrR C79S

This study

pMUPbrRC114S/PpbrA

As pMUPbrRPpbrA, but PbrR C114S

This study

pMUPbrRC132S/PpbrA

As pMUPbrRPpbrA, but PbrR C132S

This study

pMUPbrRC134S/PpbrA

As pMUPbrRPpbrA, but PbrR C134S

This study

pMUPbrRC132,134 S/PpbrA

As pMUPbrRPpbrA, but PbrR C132S/C134S

This study

pUC21

Ap^r, high copy number cloning vector; ColE1 replicon

[34]

pUK21

Km^r, intermediate copy number cloning vector; p15A replicon

[34]

pUK21pbr1

Km^r, HindIII/SalI pbrR/PpbrA/ΔpbrA from pMOL1139 cloned into pUK21

This study