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Figure 2 | BMC Microbiology

Figure 2

From: In vitro and in vivo silencing of plasmodial dhs and eIf-5a genes in a putative, non-canonical RNAi-related pathway

Figure 2

A) Silencing of parasitic EIF-5A by RNAi in 293 T cells and subsequent monitoring by RT-PCR. A cotransfection was performed with: 1) no transfected DNA; 2) recombinant, plasmodial eIF-5A expression plasmid with the 483 bp cDNA; 3) EIF-5A-shRNA construct P#18; 4) aquaporin-5-specific siRNA. B) The 983 bp GAPDH amplificate was used as an internal control in the transfected mammalian cell line.

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