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Figure 4 | BMC Microbiology

Figure 4

From: A quantitative real-time RT-PCR assay for mature C. albicans biofilms

Figure 4

Comparison of the XTT and qRT-PCR assays in the assessment of early biofilm toxicity. Candida cells were seeded at 105 cells per 30 mm2 of well surface area and were incubated for 3 h at 37°C prior to exposure to amphotericin B (4 μg/ml, 4 h) or heat (100°C, 1 h). Dark bars represent % biofilm damage, calculated from change in mean OD450 values (XTT assay) and light bars represent % damage calculated from change in mean EFB1 transcript copy numbers (qRT-PCR assay) under each experimental condition, as described in the text. Error bars represent standard deviations from three independent experiments.

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