Table 2 PCR primers
From: A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays
Gene annotation | Locus | Sequence* | Annealing temperature |
|---|---|---|---|
histone H2B | GL50803_121046 | F:CGCCTGATGAAGAAGACG R:GTGTTCCGCTTGCTGA | 60 |
14-3-3 protein | GL50803_6430 | F:CGGTATGGAAGGCGAGCT R:GCTTGAGGATGTCGTTGC | 61 |
Giardia troph antigen GTA-1 | GL50803_17090 | F:GCCCGTAGAGTTCTGG R:CGTCACTATCTCCCCG | 61 |
ubiquitin | GL50803_7110 | F:GTTGAGCCCACAGATACC R:GTTACCACCACGGAGG | 61 |
β-giardin | GL50803_4812 | F: ATGTTCACCTCCACCC R: CGGAAGTTTGCAGCCA | 62 |
centrin | GL50803_6744 | F: GCAAACCAAACGCTCG R: CCAGACGTATCCACCTC | 61 |
α-tubulin | GL50803_103676 | F: CAAGTACATGGCGTGCTGCATGAT R:TAGTTGATGCCGACCTTGAAGCCT | 61 |
SALP-1 | GL50803_4410 | F: CCGCGCCGACCCCACG R: GCTCATCCAGCATCTTGTCC | 61 |
endothelin-converting enzyme 2 | GL50803_4349 | F:CATATCACCTTCCTGA R:GACCTGGGAGACATCAATGG | 61 |
mitotic spindle checkpt. MAD2 | GL50803_100955 | F:GGCTACCCAGACCAAG R:CCCGCCTATCGGAAGA | 61 |