Figure 2From: Integration Host Factor (IHF) binds to the promoter region of the phtD operon involved in phaseolotoxin synthesis in P. syringaepv. phaseolicola NPS3121Gel shift assays with crude extracts of different pathovars of P. syringae. Radiolabeled P phtD fragment was incubated with protein extracts of P. syringae pv. phaseolicola strains NPS3121and CLY233, and P. syringae pv. tomato DC3000, grown at 18°C and 28°C in M9 minimal medium. Gel shift assays were carried out under conditions similiar to those used with crude extracts of the wild-type strain. The arrow indicates the DNA-protein complex.Back to article page