From: Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1
Strains | Phenotype/genotype | Reference |
---|---|---|
Herbaspirillum seropedicae | Â | |
SmR1 | Wild type, Nif+, SmR | [38] |
LNglnK | SmR1 containing glnK::sacB- KmR | this work |
LNglnKdel | SmR1 containing ΔglnK | this work |
LNglnB | SmR1 containing glnB::TcR | this work |
LNamtBlacZ | SmR1 containing amtB::lacZ-KmR | this work |
LNglnKamtBlacZ | LNglnKdel containing amtB::lacZ-KmR | this work |
LNglnBamtBlacZ | LNglnB containing amtB::lacZ-KmR | this work |
B12-27 | SmR1 containing glnB::Tn5-20B | [14] |
Escherichia coli | Â | Â |
DH10B | Smr; F' [proAB+ lacZΔM15] | Life Technologies |
S17.1 | SmR, Tra+ pro thi recA hsdR (RP4-2 kan::Tn7 tet::Mu) | [39] |
Plasmids | Relevant characteristics | Reference |
pACB192 | 1.7 kb DNA fragment containing the glnB gene of H. seropedicae in pSUP202 | This work |
pACB194 | glnB gene of H. seropedicae with a tetracycline resistance transposon EZ::TNâ„¢ < TET-1 > (Epicentre) in pSUP202 | this work |
pACB210 | glnB gene of H. seropedicae in pLAFR3.18Cm | this work |
pDK6 | Expression vector/tac promoter, KmR | [37] |
pDK6nifACT | H. seropedicae nifA deleted of 606 bp in the 5'coding region cloned into pDK6 carrying the nifA promoter | this work |
pDK6pnifA | nifA gene promoter region of H. seropedicae in pDK6 | this work |
pEMS140 | nifB-lacZ transcriptional fusion of H. seropedicae in pPW452 | [21] |
pEMS301 | 1.7 kb EcoRI fragment that contains the promoter region and part of the nifA gene of H. seropedicae in pTZ19R | [40] |
pLAFR3.18Cm | TcR, CmR, IncP cosmid with the pTZ18R cloning nest | [15] |
pLNΔNifA | Expresses ΔN-NifA of H. seropedicae with its own promoter in pLAFR3.18Cm | this work |
pLNOGA | 5.1 kb fragment that contains the nlmAglnKamtB operon of H. seropedicae in pLAFR3.18Cm (former named pLARF3.18OGA) | [15] |
pLNglnK | 0.9 kb BamHI/HindIII fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and 5' terminal of the amtB gene of H. seropedicae in pTZ18R | this work |
pMH1701 | KmR, contains a sacB-KmR cassette | [35] |
pPW452 | TcR, transcriptional lacZ gene fusion | [41] |
pRAM2T7 | contains H. seropedicae nifA deleted of 606 bp in the 5'end, encoding an N-truncated form of NifA deleted of its N-terminal domain and Q-linker | this work |
pRAMM1 | nifA of H. seropedicae in pLAFR3.18Cm | this work |
pRW1 | nifA-lacZ transcriptional fusion of H. seropedicae in pPW452 | [20] |
pSUP202 | ApR, CmR, TcR, Mob | [39] |
pSUPamtBClacZ | Central region of the amtB gene with a lacZ-KmR cassette insertion in pSUP202 | [15] |
pSUPglnK | 0.9 kb BamHI/HindIII fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and 5' terminal of the amtB gene of H. seropedicae in pSUP202 | this work |
pSUPglnKdel | ΔglnK (192bp) gene of H. seropedicae in pSUP202 | this work |
pSUPglnKdelsacB | contains ΔglnK and a sacB-KmR cassette (from pMH1701) cloned into the vector pSUP202 | this work |
pSUPglnKsacB | 0.9 kb fragment spanning from the 3'end of nlmA to the 5'end of amtB with a sacB-KmR (from pMH1701) inserted into the glnK gene | this work |
pTZ19R | ApR lacZ f1 IG | [42] |
pUC18 | ApR, lacZ, f1 | Invitrogen |
pUCG08del | 0.8 kb DNA fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and the 5' terminal of the amtB gene of H. seropedicae in pUC18. | this work |
pUCglnKdel | ΔglnK gene of H. seropedicae in pUC18 | this work |