From: Contribution of SecDF to Staphylococcus aureusresistance and expression of virulence factors
Strain | Relevant genotype or phenotype | Ref. or source |
---|---|---|
S. aureus | ||
Newman | Clinical isolate (ATCC 25904), rsbU+ | [64] |
RN4220 | NCTC8325-4 r- m+ | [65] |
CQ33 | NewmanΔsa2056 | This study |
CQ39 | Newman pME2, Tcr, Mcr | This study |
CQ65 | NewmanΔsa2339 | This study |
CQ66 | NewmanΔsecDF | This study |
CQ69 | NewmanΔsecDF pME2, Tcr, Mcr | This study |
CQ85 | Newman pCN34, Kmr | This study |
CQ86 | Newman pCN34 pME2, Kmr, Tcr, Mcr | This study |
CQ87 | NewmanΔsecDF pCN34, Kmr | This study |
CQ88 | NewmanΔsecDF pCN34 pME2, Kmr, Tcr, Mcr | This study |
CQ89 | NewmanΔsecDF pCQ27, Kmr | This study |
CQ90 | NewmanΔsecDF pCQ27 pME2, Kmr, Tcr, Mcr | This study |
E. coli | ||
DH5α | Cloning strain, [F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-] | Invitrogen |
Plasmid | Relevant genotype or phenotype | Reference or source |
pCN34 | S. aureus-E. coli shuttle vector, pT181-cop-wt repC aphA-3 ColE1 Kmr | [56] |
pCQ27 | pCN34 derivative carrying secDF and its promoter (Newman), Kmr | This study |
pCQ30 | pKOR1 derivative carrying 1 kb fragments of the region up- and downstream of sa2056 amplified from Newman, ligated together with EcoRI and recombined at the attP sites, Apr, Cmr | This study |
pCQ31 | pKOR1 derivative carrying 1 kb fragments of the region up- and downstream of sa2339 amplified from Newman, ligated together with HindIII and recombined at the attP sites, Apr, Cmr | This study |
pCQ32 | pKOR1 derivative carrying 1 kb fragments of the region up- and downstream of secDF amplified from Newman, ligated together with HindIII and recombined at the attP sites, Apr, Cmr | This study |
pKOR1 | E. coli-S. aureus shuttle vector used to create markerless deletions; repF(Ts) cat attP ccdB ori ColE1 bla P xyl /tetO secY570, Apr, Cmr | [23] |
pME2 | pBUS1 derivative carrying mecA and its promoter (COLn), Tcr, Mcr | [28] |