From: Production, purification, sequencing and activity spectra of mutacins D-123.1 and F-59.1
Step | Volume (mL) | Activity (AU/mL) | Total Protein (mg) | Total activity (AU.103) | Specific activity (AU/mg) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|---|---|
mutacin F-59.1 | Â | Â | Â | Â | Â | Â | Â |
Culture supernatant | 1000 | 400 | 10000 | 400 | 40 | 100 | 1 |
Sep-Pak C18 | 95 | 3200 | 3000 | 304 | 101 | 76 | 2.5 |
C18 RP-HPLC | 2 | 16000 | 0.1 | 32 | 3.2 × 105 | 8 | 8 × 103 |
mutacin D-123.1 | Â | Â | Â | Â | Â | Â | Â |
Culture supernatant | 675 | 200 | 4320 | 135 | 31 | 100 | 1 |
Sep-Pak C18 | 50 | 1600 | 8 | 80 | 1 × 104 | 59 | 320 |
C18 RP-HPLC | 1 | 800 | 0.005 | 0.8 | 1.6 × 105 | 0.2 | 5120 |