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Table 1 Purification of mutacins F-59.1 and D-123.1 by hydrophobic chromatography.

From: Production, purification, sequencing and activity spectra of mutacins D-123.1 and F-59.1

Step

Volume

(mL)

Activity

(AU/mL)

Total Protein

(mg)

Total activity

(AU.103)

Specific activity

(AU/mg)

Yield

(%)

Purification

(fold)

mutacin F-59.1

       

Culture supernatant

1000

400

10000

400

40

100

1

Sep-Pak C18

95

3200

3000

304

101

76

2.5

C18 RP-HPLC

2

16000

0.1

32

3.2 × 105

8

8 × 103

mutacin D-123.1

       

Culture supernatant

675

200

4320

135

31

100

1

Sep-Pak C18

50

1600

8

80

1 × 104

59

320

C18 RP-HPLC

1

800

0.005

0.8

1.6 × 105

0.2

5120