Table 1 Sequence analysis of rpoS in twenty-two ECOR strains
Strain | arpoS PCR fragment size | bChange in nucleotide sequence | bChange in amino acid sequence |
|---|---|---|---|
ECOR02 | 1.3 Kb | C97G | Q33E |
ECOR05 | 1.3 Kb | C97G,C942T | Q33E |
ECOR08 | 1.3 Kb | C97G,C942T | Q33E |
ECOR17 | 1.3 Kb | C97G, G377T, C942T | Q33E, G126V |
ECOR18 | 1.3 Kb | C97G, ΩT392, C942T | Q33E, E132R, K133E, F134V, D135 amber * |
ECOR20 | 1.3 Kb | T32G, C97G, C942T | L11 amber, Q33E * |
ECOR22 | 1.3 Kb | C97G, C777T, C942T | Q33E |
ECOR28 | 4.2 Kb | ΩA269 | Frameshift after aa R85 * |
ECOR32 | 4.2 Kb | C97G,G598T | Q33E, E200amber * |
ECOR33 | 4.2 Kb | C97G, ΩA after nt494, ΩT after nt915 | Q33E, frameshift after I165 * |
ECOR45 | 4.2 Kb | ΩA518 | Frameshift after aa 174 * |
ECOR50 | 4.2 Kb | C264T, T270C, T357G, T462C, T549C, G564A, T573C, G819A | wild type |
ECOR51 | 3.4 Kb | ΩT76, C97G,T163C, C264T, T357G, T462C, T573C, C732T, G819A, C987T | D26 amber * |
ECOR54 | 3.4 Kb | ΩA after nt83, C97G, T163C, C264T, T357G, T462C, T573C, C732T, G819A, C987T | Q33E, frameshift after K28** |
ECOR55 | 3.4 Kb | C97G, T163C, C264T, T357G, T462C, T573C, C732T, G819A, C987T | Q33E |
ECOR56 | 3.4 Kb | C97G, T163C, T357G, G377A, T462C, T573C, C732T, G819A, C987T | Q33E, G126E |
ECOR58 | 4.2 Kb | C97G, C672T | Q33E |
ECOR59 | 3.4 Kb | C97G, G124T, T163C, T339C, T357G, C405T, T462C, T573C, C732T | Q33E, E42 amber and frameshift after aa S186 * |
ECOR63 | 3.4 Kb | C97G, T163C, T357G, C405T, T462C, T573C, C732T, G990A | Q33E |
ECOR66 | 3.4 Kb | C97G, T163C, T357G, C421T, T462C, T573C, C732T | Q33E, R141C |
ECOR69 | 4.2 Kb | C97G | Q33E |
ECOR70 | 1.3 Kb | Δnt94-nt121 (28nts) | Δaa32-41 (10aas) * |