Table 1 List of strains, plasmids, and phage used in this study
From: Analysis of the ArcA regulon in anaerobically grown Salmonella enterica sv. Typhimurium
Strain, Plasmid, or Phage | Relevant Characteristics | Source and/or Reference |
|---|---|---|
Strains | ||
SalmonellaTyphimurium | Â | Â |
   14028s | Wild-type | American Type Culture Collection |
   TT17442/SL3052 | (LT2) containing metE205 ara-9 cob-24::MudJ arcA201::Tn10d-Tet | [21/S. Libby] |
   NC980 | 14028 s containing arcA::Tn10 (Tetr) [TT17442 (P22) × 14028s] | This study |
   NC989 | Same as NC980, but harboring parcA. | This study |
Escherichia coli | Â | Â |
   ER2420 | Harboring pACYC177 | New England Biolabs |
   ER2925 | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 | New England Biolabs |
Plasmids | ||
   pACYC177 | F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101 | New England Biolabs |
   parcA | An 897 base pair arcA amplicon from S. Typhimurium 14028 s cloned into the SmaI site within the Kanr gene of pACYC177. | This study |
Phage | ||
   P22 |  | S. Libby/Collection stock of NC |