Figure 3From: Purification and biochemical properties of a cytochrome bc complex from the aerobic hyperthermophilic archaeon Aeropyrum pernixHeme analysis by MALDI-TOF mass spectrometry of partially purified cytochrome oa 3 oxidase from A. pernix. Heme was extracted from the oxidase preparation by shaking vigorously with acetone-HCl, followed by extraction with ethyl acetate. The extracted heme was analyzed by MALDI-TOF mass spectrometry as detailed in the "Materials and Methods".Back to article page