Figure 3
Subcellular localization of TbrPPX1. Panels A-C: procyclic forms. Panels D-F: bloodstream forms. Panels A and D: c-Myc-tagged TbrPPX1; panels B and E: acidocalcisomes visualized by the VH+-PPase-antibody; panels C and F: overlay, including DAPI staining. Panel G: Detergent fractionation of bloodstream forms and procyclic cells. Pellets (P) and supernatant fractions (SN) of cells solubilized either with RIPA buffer or with 0.5% Triton X-100. Western blots were developed with monoclonal anti-c-Myc antibody (= TbrPPX1), a polyclonal antiserum against BIP, and a polyclonal antiserum against a major paraflagellar rod (PFR) protein.