Table 2 Primers used in this study
From: pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes
Name | Sequence 5' → 3' | Usage | Reference |
|---|---|---|---|
ARB6 | GGCACGCGTCGACTAGTACNNNNNNNNNNACGCC | PCR round 1 | [59] |
ARB2 | GGCACGCGTCGACTAGTAC | PCR round 2 | [59] |
ME-O-extF | CGGTTTACAAGCATAACTAGTGCGGC | PCR round 1 | This work |
ME-O-intF | AGAGGATCCCCGGGTACCGAGCTCG | PCR round 2/sequencing | This work |
ME-I-extR | CTCGTTTCACGCTGAATATGGCTC | PCR round 1 | This work |
ME-I-intR | CAGTTTTATTGTTCATGATGATATA | PCR round 2/sequencing | This work |
GFP-extR | GGGTAAGTTTTCCGTATGTTGCATC | PCR round 1 | This work |
GFP-intR | GCCCATTAACATCACCATCTAATTC | PCR round 2/sequencing | This work |