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Figure 4 | BMC Microbiology

Figure 4

From: Repression of btuB gene transcription in Escherichia coliby the GadX protein

Figure 4

Binding of GadX to btuB promoter. 32P-labeled DNA fragments PbtuB, PgadA, PgadB, and Ppal containing the promoters of btuB, gadA, gadB, and pal, respectively, were incubated with GadX fused to the maltose binding protein (MalE-GadX) at 0, 2, 4, or 6 pmoles. The reaction mixtures were electrophoresed in a 5% native polyacrylamide gel. Band shift due to GadX binding was visualized by autoradiography. Arrows indicate bands of DNA probes not bound by GadX.

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