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Table 3 Kinetics parameters for penicillin and ampicillin

From: Enzymatic analysis of the effect of naturally occurring Leu138Pro mutation identified in SHV β-lactamase on hydrolysis of penicillin and ampicillin

 

penicillin

ampicillin

Enzymes

Km(μM)

Kcat (s-1)

Kcat/Km

(μM-1s-1)

Km(μM)

Kcat (s-1)

Kcat/Km

(μM-1s-1)

SHV-1

49

1460

29.79

26

5910

227.3

SHV-1(L138P)

76

3370

4.43

87

1363

15.66

SHV-33

59

2140

36.27

16

1375

85.93

SHV33-L138P

91

2680

29.45

90

1503

16.7