From: Enzymatic analysis of the effect of naturally occurring Leu138Pro mutation identified in SHV β-lactamase on hydrolysis of penicillin and ampicillin
penicillin
ampicillin
Enzymes
Km(μM)
Kcat (s-1)
Kcat/Km
(μM-1s-1)
SHV-1
49
1460
29.79
26
5910
227.3
SHV-1(L138P)
76
3370
4.43
87
1363
15.66
SHV-33
59
2140
36.27
16
1375
85.93
SHV33-L138P
91
2680
29.45
90
1503
16.7