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Table 4 E. coli strains and plasmids used in this study

From: Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR

 

Relevant genotype

Source or construction

E. coli strains

BW27784

Δ(araBAD)567 Δ(rhaBAD)568

Yale E. coli Genetic Stock Center

 

Δ(araFGH) Φ(ΔaraEpPCP18-araE)

[32]

BW117N

BW27784 with chromosomally integrated YpTOP1-D117N gene

[10]

AQ4335

Δara leu7697 NBRP

NBRP-E. coli at NIG

FB20344

MG1655 ydeA::Tn5KAN-I-SceI

U. Wisconsin [34]

YT103

AQ4335 ydeA::Tn5KAN-I-SceI

P1(FB20344) × AQ4335, Kanr

JW1328-1

Δfnr771::kan

Yale E. coli Genetic Stock Center [35]

JW1650-1

ΔpurR746::kan

Yale E. coli Genetic Stock Center [35]

IFL6

BW27784 Δ fnr771::kan

P1(JW1328-1) × BW27784, Kanr

IFL7

BW27784 Δ purR746::kan

P1(JW1650-1) × BW27784, Kanr

Plasmids

pAYTOP128

Mutant derivative of pAYTOP encoding YpTOP1 with G122S, M326V and A383P mutations

[11]

pCRII

High copy number cloning vector

Invitrogen

pAQ5

pCR-XL-TOPO cloning product of E. coli chromosome fragment 2618398-2620765

This study

pAQ5-1

pCR-XL-TOPO carrying upp gene and the intergenic region of upp-purMN

This study

pAQ5-2

pCR-XL-TOPO carrying purM gene and the intergenic region of upp-purMN

This study

pInter

pCR-XL-TOPO carrying the intergenic region of upp-purMN

This study

pInterD1

pInter with the FNR binding site deleted

This study

pInterD2

pInter with the PurR binding site deleted

This study