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Table 1 Integrity of antibiotic-treated bacteria

From: Contribution of bacterial outer membrane vesicles to innate bacterial defense

  

WC

(ng/mL)

OMV-free Supe

(ng/mL)

AP Leakage

([AP] supe :[AP] whole cell ) a

Strain

Treatmentb

UNT

TRE

UNT

TRE

Untreated

Treated

MK318

Polymyxin B

(0.75 μg/mL)

8.270 ± 1.010

7.870 ± 0.970

1.290 ± 0.080

1.341 ± 0.121

0.160 ± 0.007

0.170 ± 0.006

 

Colistin

(0.5 μg/mL)

9.440 ± 0.230

8.87 ± 0.07

1.20 ± 0.010

1.260 ± 0.021

0.127 ± 0.003

0.121 ± 0.002

ETEC

Polymyxin B

(3 μg/mL)

6.100 ± 0.440

6.07 ± 0.510

1.201 ± 0.030

1.22 ± 0.030

0.198 ± 0.009

0.204 ± 0.020

ADA600

Untreated

0.020 ± 0.011

ND

0.024 ± 0.013

ND

ND

ND

  1. aRFU measurements of AP in the OMV-free culture supernatant (Supe) compared to AP in whole cell (WC) pellets, normalized to CFU/mL in the culture. No significant differences in AP leakage between untreated (UNT) and treated (TRE) cultures were observed (p > 0.05).
  2. bTreatments were for 2 h at 37°C; final concentration of treatments are shown. (n = 9)