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Table 1 Taxonomic identification and phylogenetic affiliation of the excised and sequenced epiphytic (EP), washing water (WW) and cultivation water (CW) DGGE bands

From: Life without a cell membrane: Challenging the specificity of bacterial endophytes within Bryopsis (Bryopsidales, Chlorophyta)

DGGE band number

Closest matching strain in BLAST (accession number)

Query coverage/Maximum identity

Phylogenetic affiliation

Correlation

MX19 EP 1

Uncultured Mycoplasma sp. clone MX19.9 (JF521606) 100/100

Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae

M1m + M1b -

MX19 EP 2

Uncultured bacterium clone Del10081H12 (JF262029) 100/100

Proteobacteria; Alphaproteobacteria; Caulobacterales; Hyphomonadaceae

M4 -

MX19 EP 3

Uncultured Phyllobacteriaceae bacterium clone MX19.12 (JF521607) 100/100

Proteobacteria; Alphaproteobacteria; Rhizobiales; Phyllobacteriaceae

M8 +

MX19 EP 4

Uncultured bacterium isolate TTGE gel band N68 (JN185170) 100/100

Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae

M9 -

MX19 EP 5

Uncultured Labrenzia sp. clone DGGE band C (HE599215) 100/100

Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae

M10 +

MX90 EP 6

Uncultured bacterium clone CD02003D03 (HM768522) 100/96

Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae

M5 -

MX90 EP 7

Uncultured Phyllobacteriaceae bacterium clone MX19.12 (JF521607) 100/100

Proteobacteria; Alphaproteobacteria; Rhizobiales; Phyllobacteriaceae

M8 +

MX90 EP 8

Uncultured alphaproteobacterium clone TH_d327 (EU272970) 100/98

Proteobacteria; Alphaproteobacteria; Rhizobiales, Hyphomicrobiaceae

M9 -

MX90 WW 9

Uncultured bacterium clone OTU017 (GU174663) 100/100

Proteobacteria; Alphaproteobacteria; Rhizobiales; Bartonellaceae

M2 -

MX164 EP 10

Uncultured Mycoplasma sp. clone MX19.9 (JF521606) 100/96

Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae

M1m + M1b -

MX164 EP 11

Uncultured Arcobacter sp. clone MX164.20 (JF521610) 100/100

Proteobacteria; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae

M2 +

MX164 EP 12

Uncultured proteobacterium clone Marsh_0_33 (JF980756) 100/100

Proteobacteria; Alphaproteobacteria; Caulobacterales; Hyphomonadaceae

M3 -

MX164 EP 13

Acanthopleuribacter pedis type strain NBRC 101209 (AB303221) 100/93

Acidobacteria; Holophagae; Acanthopleuribacterales

M5 -

MX164 EP 14

Hyphomicrobiaceae bacterium WPS10 (HQ638980) 100/98

Proteobacteria; Alphaproteobacteria; Rhizobiales; Bartonellaceae

M8 -

MX164 EP 15

Uncultured bacterium clone I3A_12H (EU352599) 100/98

Proteobacteria; Alphaproteobacteria; Rhizobiales; Methylobacteriaceae

M9 -

MX164 EP 16

Stappia sp. enrichment culture clone NKiNSO2 (EU983274) 100/95

Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae

M10 -

MX164 WW 17

Uncultured Sneathiella sp. clone w-G7 (HQ727092) 100/97

Proteobacteria; Alphaproteobacteria; Sneathiellales; Sneathiellaceae

M7 -

MX263 EP 18

Thalassomonas sp. UST061013-012 (EF587959) 100/100

Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae

M7 -

MX263 EP 19

Uncultured Phyllobacteriaceae bacterium clone MX19.12 (JF521607) 100/100

Proteobacteria; Alphaproteobacteria; Rhizobiales; Phyllobacteriaceae

M8 +

MX263 EP 20

Uncultured Labrenzia sp. clone DGGE band C (HE599215) 100/100

Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae

M10 +

MX263 WW 21

Uncultured Mycoplasma sp. clone MX263.1 (JF521605) 100/100

Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae

M1m + M1b -

MX263 CW 22

Uncultured bacterium isolate DGGE gel band B12 (HQ875697) 100/93

Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae

M3 -

MX263 CW 23

Alcanivorax dieselolei strain PM07 (HM596594) 100/100

Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae

M6 -

MX344 EP 24

Uncultured Labrenzia sp. clone DGGE band C (HE599215) 100/100

Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae

M10 +

MX344 WW 25

Ruegeria mobilis strain F4122 (HQ338148) 100/99

Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae

M8 -

MX344 CW 26

Uncultured bacterium clone EMar8 (FR667032) 100/94

Proteobacteria; Gammaproteobacteria; Alteromonadales

M4 -

MX344 CW 27

Uncultured bacterium clone W2-97 (HQ322761) 100/90

Proteobacteria; Alphaproteobacteria

M7 -

  1. The band numbers correspond to the numbers (1-27) in Figure 4. The last column shows the correlation (positive + or negative -) between the identification of a band and the sequence information of the marker band (M1m, M1b, M2-M10) at the same position.