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Table 1 Comparison of Protein Expression Profiles during Biofilm and Planktonic Growth

From: Biofilm and planktonic pneumococci demonstrate disparate immunoreactivity to human convalescent sera

   

Detected Peptides

Group and Function

Protein

Gene

Biofilm

Planktonic

Protein synthesis and processing

30S ribosomal protein S2

rpsB (SP_2215)

14

22

 

30S ribosomal protein S3

rpsC (SP_0215)

4

5

 

30S ribosomal protein S4

rpsD (SP_0085)

4

5

 

30S ribosomal protein S5

rpsE (SP_0227)

6

23

 

30S ribosomal protein S8

rpsH (SP_0224)

6

10

 

30S ribosomal protein S7

rpsG (SP_0272)

0

3

 

30S ribosomal protein S10

rpsJ (SP_0208)

3

4

 

30S ribosomal protein S12

rpsL (SP_0271)

0

3

 

30S ribosomal protein S11

rpsK (SP_0235)

4

6

 

30S ribosomal protein S13

rpsM (SP_0234)

0

2

 

30S ribosomal protein S17

rpsQ (SP_0218)

0

4

 

50S ribosomal protein L1

rplA (SP_0631)

9

28

 

50S ribosomal protein L2

rplB (SP_0212)

0

7

 

50S ribosomal protein L3

rplC (SP_0209)

0

4

 

50S ribosomal protein L4

rplD (SP_0210)

5

11

 

50S ribosomal protein L5

rplE (SP_0221)

10

23

 

50S ribosomal protein L6

rplF SP_0225

6

5

 

50S ribosomal protein L7/L12

rplL (SP_1354)

0

14

 

50S ribosomal protein L9

rplI (SP_2204)

0

2

 

50S ribosomal protein L10

rplJ (SP_1355)

0

7

 

50S ribosomal protein L11

rplK (SP_0630)

0

9

 

50S ribosomal protein L14

rplN (SP_0219)

3

5

 

50S ribosomal protein L15

rplO SP_0229

0

5

 

50S ribosomal protein L18

rplR (SP_0226)

0

3

 

50S ribosomal protein L19

rplS (SP_1293)

8

8

 

50S ribosomal protein L21

rplU (SP_1105)

0

2

 

50S ribosomal protein L22

rplV (SP_0214)

0

6

 

50S ribosomal protein L30

rpmD (SP_0228)

0

2

 

Elongation factor G

fusA (SP_0273)

10

72

 

Elongation factor P

efp (SP_0435)

5

7

 

Elongation factor Ts

tsf (SP_2214)

14

16

 

Elongation factor Tu

(SP_0681)

29

59

 

Arginyl-tRNA synthetase

argS (SP_2078)

11

14

 

Alanyl-tRNA synthetase

alaS (SP_1383)

4

8

 

Glutamyl-tRNA(Gln) amidotransferase subunit A

gatA (SP_0437)

0

4

 

Glycyl-tRNA synthetase alpha subunit

glyQ (SP_1475)

0

4

 

Methionyl-tRNA formyltransferase

fmt (SP_1735)

0

2

 

Methionyl-tRNA synthetase

metG (SP_0788)

0

2

 

Phenylalanyl-tRNA synthetase beta chain

pheT (SP_0581)

0

2

 

Prolyl-tRNA synthetase

proS (SP_0264)

0

12

 

10 kDa chaperonin

groES (SP_1907)

3

8

 

60 kDa chaperonin

groEL (SP_1906)

24

57

 

33 kDa chaperonin

hslO (SP_2188)

0

4

 

Chaperone protein dnaK

dnaK (SP_0517)

12

89

 

ATP-dependent Clp protease ATP-binding subunit

clpE (SP_0820)

3

3

 

ATP-dependent Clp protease proteolytic subunit

clpP (SP_0746)

0

5

 

Adapter protein mecA

mecA SP_1362

0

2

 

Ribosome-recycling factor

frr (SP_0945)

0

2

 

Foldase protein prsA

prsA SP_0981

13

5

 

Peptide chain release factor 3

prfC (SP_0439)

4

0

 

Peptide deformylase

def (SP_1456)

0

4

 

Protein grpE

grpE (SP_0516)

0

5

Energy Metabolism

Phosphoglycerate kinase

pgk (SP_0499)

22

46

 

L-lactate dehydrogenase

ldh (SP_1220)

21

30

 

Glyceraldehyde-3-phosphate dehydrogenase

gapN (SP_1119)

10

38

 

Fructose-bisphosphate aldolase

fba SP_0605

6

32

 

Glycerol-3-phosphate dehydrogenase [NAD(P)+]

gpsA (SP_2091)

2

6

 

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase

gpmA (SP_1655)

7

11

 

6-phosphofructokinase

pfkA (SP_0896)

8

21

 

Phosphoenolpyruvate-protein phosphotransferase

ptsI (SP_1176)

0

10

 

Ribose-phosphate pyrophosphokinase 1

prsA (SP_0027)

2

4

 

Ribose-5-phosphate isomerase A

rpiA (SP_0828)

0

3

 

Triosephosphate isomerase

tpiA (SP_1574)

2

8

 

Tagatose 1,6-diphosphate aldolase

lacD (SP_1190)

4

6

 

Phosphoenolpyruvate-protein phosphotransferase

ptsI (SP_1176)

0

6

 

Ribose-phosphate pyrophosphokinase 2

prs2 (SP_1095)

0

3

 

Phosphoglucosamine mutase

glmM (SP_1559)

0

2

 

Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]

glmS (SP_0266)

12

31

 

Ornithine carbamoyltransferase, catabolic

arcB (SP_2150)

4

2

 

Dihydrodipicolinate reductase

dapB (SP_1555)

0

4

 

Dihydrodipicolinate synthase

dapA (SP_1014)

0

3

 

Glucosamine-6-phosphate deaminase

nagB (SP_1415)

0

2

 

Carbamoyl-phosphate synthase large chain

carB (SP_1275)

5

5

 

2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase

dapH (SP_2097)

0

8

 

Aspartate--ammonia ligase

asnA (SP_1970)

3

0

 

Dihydroxy-acid dehydratase

ilvD (SP_2126)

0

7

 

ATP synthase subunit alpha

atpA (SP_1510)

4

21

 

ATP synthase subunit beta

atpD (SP_1508)

9

18

 

ATP synthase gamma chain

atpG (SP_1509)

0

8

 

Phosphate import ATP-binding protein PstB 1

pstB1 (SP_1396)

0

9

 

Phosphate import ATP-binding protein PstB 2

pstB2 (SP_1397)

0

4

 

Maltose/maltodextrin-binding protein

malX (SP_2108)

0

10

 

Manganese ABC transporter substrate-binding lipoprotein

psaA (SP_1650)

13

0

 

GMP synthase [glutamine-hydrolyzing]

guaA (SP_1445)

6

21

 

Hypoxanthine-guanine phosphoribosyltransferase

guaA (SP_1445)

0

9

 

Adenylate kinase

guaA (SP_1445)

2

5

 

Inosine-5'-monophosphate dehydrogenase

guaA (SP_1445)

0

14

 

Uracil phosphoribosyltransferase

guaA (SP_1445)

4

11

 

Dihydroorotate dehydrogenase

guaA (SP_1445)

0

5

 

Uridylate kinase

guaA (SP_1445)

0

5

 

CTP synthase

guaA (SP_1445)

3

0

 

Bifunctional protein glmU

guaA (SP_1445)

0

3

 

Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta

accD (SP_0426)

0

2

 

Phosphate acyltransferase

plsY (SP_0851)

0

2

 

Formate--tetrahydrofolate ligase

fhs (SP_1229)

0

5

 

6,7-dimethyl-8-ribityllumazine synthase

ribH (SP_0175)

0

3

 

manganese-dependent inorganic pyrophosphatase

ppaC (SP_1534)

2

3

 

Serine hydroxymethyltransferase

glyA (SP_1024)

0

7

 

Pyridoxal biosynthesis lyase pdxS

pdxS (SP_1468)

0

9

Capsule production & Cell wall

Tyrosine-protein kinase CpsD

cpsD (SP_0349)

0

2

 

Glucan 1,6-alpha-glucosidase

dexB (SP_0342)

2

3

 

UTP--glucose-1-phosphate uridylyltransferase

cap4C (SP_2092)

4

4

 

UDP-N-acetylmuramoylalanine--D-glutamate ligase

murD (SP_0688)

0

3

 

D-alanine--poly(phosphoribitol) ligase subunit 1

dltC (SP_2174)

0

3

Virulence Factors

Enolase

eno (SP_1128)

25

89

 

Pyruvate oxidase

spxB (SP_0730)

28

62

 

Pneumolysin

ply (SP_1923)

0

11

 

PsrP

psrP (SP_1772)

72

21

Unknown & Hypothetical

DegV domain-containing protein

(SP_1112)

0

5

 

UPF0176 protein

(SP_0095)

2

0

 

UPF0371 protein

(SP_0341)

0

6

 

UPF0082 protein

(SP_1922)

0

8

 

Probable transketolase

tkt (SP_2030)

4

37

Regulation & DNA Binding

HPr kinase/phosphorylase

hprK (SP_1413)

0

2

 

Single-stranded DNA-binding protein

ssb (SP_1540)

0

9

 

DNA-binding protein HU

hup (SP_1113)

0

10

 

GTP-sensing transcriptional pleiotropic repressor CodY

codY (SP_1584)

0

2

 

Pur operon repressor

purR (SP_1979)

0

5

Transcription

DNA-directed RNA polymerase subunit alpha

rpoA (SP_0236)

5

3

 

DNA-directed RNA polymerase subunit beta

rpoB (SP_1961)

2

4

 

Transcription elongation factor GreA

greA (SP_1517)

0

3