A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

BMC Microbiology

Table 2 Putative membrane protein complexes of C. thermocellum

Complex	Spots Id^d	Gene locus	Protein descriptions^e	Approximate mass^f(kDa)
C1	9	Cthe_2267	V-type ATP synthase subunit A	300
	10	Cthe_2268	V-type ATP synthase beta chain
C2	10	Cthe_2608	ATP synthase F1, beta subunit	300
	11	Cthe_2606	ATP synthase F1, alpha subunit
C3	1	Cthe_0423	bifunctional acetaldehyde/alcohol dehydrogenase	>880
C4	15	Cthe_0699	carboxyl transferase	220
C5	19	Cthe_1840	cysteine synthase	130
C6	27	Cthe_1869	ornithine carbamoyltransferase	100
C7	3	Cthe_2253	ATP-dependent metalloprotease FtsH	>880
C8	13	Cthe_0418	polynucleotide phosphorylase/polyadenylase	150
C9	7	Cthe_0016	Ferritin and Dps	440
C10	14	Cthe_3148	ABC transporter related protein	140
C11	16	Cthe_1020	solute-binding protein	190
	18	Cthe_1018	binding-protein-dependent transport systems inner membrane component
	21	Cthe_1862	ABC transporter related protein
C12	17	Cthe_1557	ABC transporter related protein ATP-binding protein	140
C13	22	Cthe_1754	solute-binding protein	170
C14	12	Cthe_2348	S-layer-like region; Ig-related	140
C15	20	Cthe_1104	prepilin-type cleavage/methylation	20~180

Note:
^dSpots identification numbers (Spots ID) correspond to the numbers in Figure 1.
^eProtein annotations are based on the genome annotation of C. thermocellum ATCC 27405.
^fApproximate mass observed on BN-PAGE.

ISSN: 1471-2180