Table 6 Strains and plasmids used in this study
Strains/plasmids | Genotype | Reference |
|---|---|---|
MC4100 | F- araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150 - | [37] |
DHP-F2 | MC4100 ΔhypF 59-629AA | [16] |
XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZΔM15 Tn10 (TetR)] | Stratagene |
PM06 | Like MC4100 but feoB::Tn5 | This study |
PX06 | Like XL1-Blue but feoB::Tn5 | This study |
CP411 | Like MC4100 but ΔentC::cat feoB::Tn5 | This study |
CP413 | Like MC4100 but ΔfecA-E ΔentC::cat feoB::Tn5 | This study |
CP415 | Like MC4100 but ΔfecA-E ΔentC::cat | This study |
CP416a | Like MC4100 but ΔentC::cat | This study |
CP422 | Like MC4100 but ΔfecA-E introduced from GG7 | This study |
GG7 | W3110 ΔfecA-E::kan | G. Grass |
CP971 | MC4100 ΔhycAI::kan | [38] |
CP612 | Like MC4100 but Φ(hyaA'-'lacZ) | This study |
CP775 | Like MC4100 but Φ(hybO'-'lacZ) | This study |
CP951 | Like MC4100 but Φ(hycA'-'lacZ) | This study |
CP1069 | Like MC4100 but ΔhypF Φ(hyaA'-'lacZ) | This study |
CP1084 | Like MC4100 but ΔhypF Φ(hybO'-'lacZ) | This study |
CP1149 | Like MC4100 but ΔhypF Φ(hycA'-'lacZ) | This study |
CP1073 | Like MC4100 but ΔfecA-E Φ(hyaA'-'lacZ) | This study |
CP1088 | Like MC4100 but ΔfecA-E Φ(hybO'-'lacZ) | This study |
CP1150 | Like MC4100 but ΔfecA-E Φ(hycA'-'lacZ) | This study |
CP1075 | Like MC4100 but ΔfeoBb Φ(hyaA'-'lacZ) | This study |
CP1090 | Like MC4100 but ΔfeoBb Φ(hybO'-'lacZ) | This study |
CP1151 | Like MC4100 but ΔfeoBb Φ(hycA'-'lacZ) | This study |
CP1071 | Like MC4100 but ΔentC Φ(hyaA'-'lacZ) | This study |
CP1086 | Like MC4100 but ΔentC Φ(hybO'-'lacZ) | This study |
CP1152 | Like MC4100 but ΔentC Φ(hycA'-'lacZ) | This study |
CP1079 | Like MC4100 but ΔfecA-E feoBb Φ(hyaA'-'lacZ) | This study |
CP1094 | Like MC4100 but ΔfecA-E feoBb Φ(hybO'-'lacZ) | This study |
CP1153 | Like MC4100 but ΔfecA-E feoBb Φ(hycA'-'lacZ) | This study |
CP1081 | Like MC4100 but ΔentC feoBb Φ(hyaA'-'lacZ) | This study |
CP1096 | Like MC4100 but ΔentC feoBb Φ(hybO'-'lacZ) | This study |
CP1154 | Like MC4100 but ΔentC feoBb Φ(hycA'-'lacZ) | This study |
CP1077 | Like MC4100 but ΔentC fecA-E Φ(hyaA'-'lacZ) | This study |
CP1092 | Like MC4100 but ΔentC fecA-E Φ(hybO'-'lacZ) | This study |
CP1155 | Like MC4100 but ΔentC fecA-E Φ(hycA'-'lacZ) | This study |
CP1083 | Like MC4100 but ΔentC fecA-E feoBb Φ(hyaA'-'lacZ) | This study |
CP1098 | Like MC4100 but ΔentC fecA-E feoBb Φ(hybO'-'lacZ) | This study |
CP1163 | Like MC4100 but ΔentC fecA-E feoBb Φ(hycA'-'lacZ) | This study |
Plasmids | Â | Â |
pFEO | feoABC+ from E. coli in pASK-IBA7 | [39] |
pECD 1079 | feoB+ from E. coli in pASK-IBA7 | N. Taudte and G. Grass |
pRS552 | KmR ApR lacZ+ lacY+ lacA+ | [20] |
phyaA552 | like pRS552 but containing Φ(hyaA'-'lacZ) | This study |
phybO552 | like pRS552 but containing Φ(hybO'-'lacZ) | This study |
pTL101 | like pRS552 but containing Φ(hycA'-'lacZ), cloned from PstI within hycA to AvaII within hycA | [28] |