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Table 3 Hydrogen-oxidizing enzyme activity in various transport mutants

From: Iron restriction induces preferential down-regulation of H2-consuming over H2-evolving reactions during fermentative growth of Escherichia coli

Straina and genotype

Hydrogenase Specific activityb

(μmol H2 oxidized min-1 mg protein-1)

MC4100

2.70 ± 0.8

DHP-F2 (hypF)

0.02 ± 0.01

PM06 (feoB)

1.24 ± 1.0

CP422 (fecA-E)

2.54 ± 1.6

CP416 (entC)

2.05 ± 0.5

CP411 (entC feoB)

0.58 ± 0.4

CP415 (fecA-E entC)

1.11 ± 0.4

CP413 (entC feoB fecA-E)

0.19 ± 0.16

  1. a Cell extracts were prepared from cells grown anaerobically in TGYEP plus 15 mM formate.
  2. b The mean and standard deviation of at least three independent experiments are shown.