Table 1 Bacterial strains and plasmid used in this work
Strain | Relevant characteristics | Reference |
|---|---|---|
Rhizobium etli CE3 | Streptomycin resistant derivative of CFN42 strain | [20] |
R. etli CFNX101 | recA::Ω-Spectinomycin derivative of CE3 | [46] |
R. etli CFNX107 | recA:: Ω-Spectinomycin derivative of CE3, laking plasmid p42a and p42d. | [46] |
E. coli S17-1 | Plasmid donor in conjugations | [23] |
Plasmid | Relevant characteristics | Reference |
pDOP | A chloramphenicol resistant suicide vector derived from pBC SK(+), and containing oriT | This work |
pDOP-E' | pDOP derivative with the intergenic region repB-repC, the complete repC gene under Placpromoter, and 500 pb downstream repC stop codon. | [22] |
pDOP-H3 | pDOP derivative carrying a 5.6 Kb HindIII with repABC operon of R. etli plasmid p42d. | This work |
pDOP-αC | pDOP derivative with the intergenic region repB-repC and the complete repC gene under Plac promoter. | This work |
pDOP-C | pDOP carrying repC gen of plasmid p42d, with a SD sequence (AGGA) and under Plac promoter. | This work |
pDOP-C/D1UM | Similar to pDOP-C but with a repC gene carrying a deletion from codon 2 to codon 29 | This work |
pDOP-C/RD1L | Similar to pDOP-C but with a repC gene carrying a deletion from codon 372 to codon 401 | This work |
pDOP-F1 | pDOP containing a repC fragment from codon 2 to codon 110, with a SD consensus sequence under Plac promoter. | This work |
pDOP-C/F1-F2 | pDOP containing a repC fragment from codon 2 to codon 209, with a SD consensus sequence under Plac promoter. | This work |
pDOP-C/F1-F3 | pDOP containing a repC fragment from codon 2 to codon 309, with a SD consensus sequence under Plac promoter. | This work |
pDOP-C/F4 | pDOP containing a repC fragment from codon 310 to codon 403, with a SD consensus sequence under Plac promoter. | This work |
pDOP-C/F4-F3 | pDOP containing a repC fragment from codon 210 to codon 403, with a SD consensus sequence under Plac promoter. | This work |
pDOP-C/F4-F2 | pDOP containing a repC fragment from codon 111 to codon 403, with a SD consensus sequence under Plac promoter. | This work |
pDOP-C s/SD | Similar to pDOP-C but without the SD sequence | This work |
pDOP-TtMC | Similar to pDOP-C but with a mutant repC gene carrying | This work |
silent mutations to increase its CG content | ||
pDOP-CBbglll | Similar to pDOP-C but with repC gene, carrying a frameshift mutation at the BglII restriction site | This work |
pDOP-CSphI | Similar to pDOP-C but with repC gene, carrying a frameshift mutation at the SphI restriction site | This work |
pDOP-CAtLC | pDOP derivative carrying repC gen of the Agrobacterium | This work |
tumefaciens C58 linear chromosome, with a SD sequence (AGGA) and under Plac promoter. | ||
pDOP-CsA | pDOP derivative carrying repC gen of the Sinorhizobium meliloti 1021 pSymA, with a SD sequence (AGGA) and under Plac promoter. | This work |
pDOP/C420-1209 | pDOP with a hybrid repC gene, encoding the first 140 amino acid residues of the pSymA RepC protein and the rest of p42d. | This work |
pDOP/C1-420 | pDOP with a hybrid repC gene, encoding the first 140 amino acid residues of the p42d RepC protein and the rest of pSymA. | This work |
pDOP/C421-840 | pDOP with a hybrid repC gene encoding, the first 140 amino acid residues of the pSymA RepC protein, the next 140 amino acid residues from the p42d RepC protein and the rest from the pSymA RepC protein. | This work |
pDOP/Cs421-840 | pDOP witha hybrid repC gene, encoding the first 140 amino acid residues of the p42d RepC protein, the next 140 amino acid residues from the pSymA RepC protein and the rest from the p42d RepC protein. | This work |
pDOP/C841-1209 | pDOP derivative with a hybrid repC gene, encoding the first 280 aminoacid residued of pSymA RepC and the rest of p42d RepC protein. | This work |
pDOP/C1-990 | pDOP derivative with a hybrid repC gene, encoding the first 330 amino acid residues of p42d RepC protein and the rest of pSymA RepC protein. | This work |
pDOP/C1-1086 | pDOP derivative with a hybrid repC gene, encoding the first 362 amino acid residues of p42d RepC protein and the rest of pSymA RepC protein. | This work |