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Table 3 Reactivity of MAbs with different types of treated and untreated antigens as assessed by ELISA.

From: Characterization of surface proteins of Cronobacter muytjensiiusing monoclonal antibodies and MALDI-TOF Mass spectrometry

Type of antigen **

Treatment

Absorbance (405 nm) ± SD *

  

A1

B5

2C2

C5

OMP

None

1.375 ± 0.20

0.720 ± 0.15

1.234 ± 0.58

1.481 ± 0.12

OMP

1% SDS + Boiling for 5 min

0.958 ± 0.07

0.492 ± 0.04

0.562 ± 0.08

0.901 ± 0.08

WC

None

1.365 ± 0.08

0.565 ± 0.07

0.725 ± 0.08

0.835 ± 0.03

WC

Heat

1.156 ± 0.16

0.423 ± 0.08

0.782 ± 0.03

1.026 ± 0.19

LPS

None

0.553 ± 0.08

0.454 ± 0.04

0.425 ± 0.09

0.531 ± 0.04

None

None

0.477 ± 0.05

0.469 ± 0.24

0.520 ± 0.07

0.412 ± 0.17

  1. OMP: outer membrane protein; WC: whole cell; LPS: Lipopolysaccharides, SD: Standard deviation.
  2. * Absorbance represents the average of two readings
  3. ** All antigens were prepared from C. muytjensii ATCC 51329
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BMC Microbiology

ISSN: 1471-2180

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