Strain or plasmid | Relevant genotype or characteristic | Reference or source |
---|---|---|
Strains | Â | Â |
E. coli DH5α | λ- ϕ80dlacΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK- mK-) supE44 thi-1 gyrA relA1 | [49] |
SE1457 | Biofilm positive strain | [50] |
S. aureus RN4220 | Restriction-negative, modification-positive isolate | [51] |
SE1457ΔsaeRS | saeRS deletion mutant of strain 1457, Spcr | This study |
SE1457saec | 1457ΔsaeRS complemented with saeRS | This study |
Plasmids | Â | Â |
pET-28a(+) | Expression vector, KanR | Novagen |
pBT2 pCX19 | Temperature-sensitive E. coli- Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus) Derivate of pCX15 | [52] [53] |
pMAD | Escherichia coli/Staphylococcus Shuttle vector | [54] |
pMAD-saeRS | Vector for allelic gene replacement of saeRS in S. epidermidis | This study |
pBT2-saeRS | Vector for complementation of saeRS in S. epidermidis 1457ΔsaeRS | This study |