Figure 2

Repression of flagellar biosynthesis and motility is dependent on the loss of Hha and YdgT. (A) Wild type, Δhha, ΔydgT and Δhha ΔydgT were assessed for flagellar-based motility using a 0.25% soft agar motility assay in which 2 μL of overnight culture was inoculated into semi-solid agar and incubated at 37°C for 6 h. (B) The radius of the motility halo region was quantified after 6 h and is shown as means with standard errors. (C) Bacteria and surface flagella were negatively stained using a 0.1% uranyl acetate solution and visualized using scanning transmission electron microscopy. Data represents three independent experiments.