Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms

Table 3 Identification and FISH reactivity profiles of five isolates from in sit u biofilms 013, 051 and 059

	Isolated strain (biofilm of origin)
	OMZ 1117 (013)	OMZ 1118 (013)	OMZ 1119 (051)	OMZ 1120 (051)	OMZ 1121 (059)
*16S rRNA probes*
LGC358a	2-4 +	3-4 +	3-4 +	3-4 +	3 +
LAB759 + LAB759-comp	- to 2 +	3-4 +	3-4 +	3 +	- to 2 +
Lpla759	-	-	-	-	-
Lpla990+ H1018	-	-	-	-	-
L-Lbre466	-	-	-	-	-
L-Lbuc438	-	- ^a	- ^a	- ^a	-
Lcas467	-	4 +	-	3-4 +	-
Lsal574	-	-	-	-	-
L-Lsal1113	-	-	-	-	-
Lreu986 + H967	2-4 +	-	3-4 +	-	3-4 +
Lfer466 + H448 + H484	2-4 +	-	-	-	3-4 +
L-Lcol732	-	-	-	-	-
Lvag222	-	-	3-4 +	-	-
Lgas458	-	-	-	-	-
Lgas183	-	-	-	-	-
*Identification* ^c	L. fermentum	L. paracasei L.casei	L. vaginalis	L. paracasei L.casei	L. fermentum

^a Positive at ≤ 45% formamide.
^b Scoring of fluorescence intensity is described in a footnote to Table 2.
^c Species identification was based on ≥ 99.5% 16S rRNA gene sequence similarity with reference strains listed in RDP10 (http://rdp.cme.msu.edu/index.jsp). The respective partial 16S rRNA gene sequences of OMZ 1117 and 1121 [EMBL: FR667951], and of OMZ 1118 and 1120 [EMBL: FR667952] were identical. OMZ 1119 was identified as L. vaginalis [EMBL: FR667953].

ISSN: 1471-2180