Figure 5From: Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectinBinding of L. biflexa transformants to extracellular matrix components. A. Fibronectin binding assay was performed with L. biflexa wild-type strain (wt), and ligA- (+ligA), and ligB- (+ligB) transformed L. biflexa. The means and standard deviations of triplicates from a representative of more than three independent experiments are shown, with statistical significance at P < 0.01 (*). B. Laminin, elastin, and collagen type I (Col I) and type IV (Col IV) binding was measured as in A. with P < 0.05 (#).Back to article page