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Table 2 Bacterial strains and plasmids used in this study

From: SO2426 is a positive regulator of siderophore expression in Shewanella oneidensisMR-1

Bacterial Strains

Genotype

Source/Reference

Shewanella oneidensis MR-1

Wild type

ATCC 7005500 Lab stock

MR-1/Δso2426

Deletion of so2426 locus

[21]

E. coli TOP10

Cloning and expression strain

Invitrogen

E. coli ER2508

Major proteinase-deficient strain

New England Biolabs

His-ER-2426-1-1

Expresses full-length SO2426 protein

This study

His-Top-26s-4

Expresses truncated SO2426 protein

This study

E. coli (pTOPO)

Vector-only control

Invitrogen

Plasmids

  

pTrcHis-2426sh

so2426sh cloned in frame with N-terminal polyhistidine

This study

pTrcHis-2426

so2426 cloned in frame with N-terminal polyhistidine

This study