From: SO2426 is a positive regulator of siderophore expression in Shewanella oneidensisMR-1
Bacterial Strains | Genotype | Source/Reference |
---|---|---|
Shewanella oneidensis MR-1 | Wild type | ATCC 7005500 Lab stock |
MR-1/Δso2426 | Deletion of so2426 locus | [21] |
E. coli TOP10 | Cloning and expression strain | Invitrogen |
E. coli ER2508 | Major proteinase-deficient strain | New England Biolabs |
His-ER-2426-1-1 | Expresses full-length SO2426 protein | This study |
His-Top-26s-4 | Expresses truncated SO2426 protein | This study |
E. coli (pTOPO) | Vector-only control | Invitrogen |
Plasmids | Â | Â |
pTrcHis-2426sh | so2426sh cloned in frame with N-terminal polyhistidine | This study |
pTrcHis-2426 | so2426 cloned in frame with N-terminal polyhistidine | This study |