Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli

Table 1 Examples of Ftp library clones that express adhesive polypeptides

Clone Name	Length of insert*	Chromosomal location of insert^†	ORFs^‡ in insert	Predicted gene product(s) of the Ftp-clone	Presence of FliC_1-20 and/or FLAG-tag in the gene product	Binding specificity of the product	Predicted molecular mass^#
ΔNarG	393	2465481-2465873	1) 02681	NarG ^§1	FliC _1-20 FLAG-tag	None	18.5
ΔFnBPA	346	2581863-2582208	1) 02803	FnBPA ^§2	FliC _1-20 FLAG-tag	Fn	16.6
ΔEbh	582	1398633-1399214	1) 01447	Ebh ^§2	FliC _1-20 FLAG-tag	Fn	24.2
ΔCoa	825	212434-213258	1) 00192	coagulase	FliC _1-20 FLAG-tag	Fg, Fn	34.2
ΔPurK	383	979768-980150	1) 01008	out of frame^¶	No
			2) 01009	PurK ^§1	FLAG-tag	Fn, Fg	14.6
ΔSCOR	484	2667518-2668001	1) 02897	terminator in sequence	FliC
			2) 02898	Putative SCOR ^§1	FLAG-tag	Fn, Fg	17.7
ΔUsp	664	1724620-1725283	1) 01818	out of frame^¶	No
			2) 01819	Usp ^§1 -like	FLAG-tag	Fn, Fg, CIV,	19.3
ΔIspD	885	244692-245576	1) 00223	out of frame^¶	No
			2) 00225	IspD ^§2	FLAG-tag	Fn, Fg	13.4
ΔPBP	756	2257336-2258091	1) 02433	out of frame^¶	No
			2) 02432	out of frame^¶	No
			3) 02430	putative PBP ^§1 of ABC ^§1 transporter	FLAG-tag	Fn, Fg	6.7

* In base pairs
† In S. aureus subsp. aureus NCTC 8325
‡ Open reading frames (ORFs) in the clones are partial, the number refers to the systematic gene identifier SAOUHSC_no. in the GenBank database, a locus_tag
§1 Abbreviations of TIGR Family names: NarG, nitrate reductase α-subunit; PurK, Phosphoribosylamino-imidazole carboxylase ATPase subunit; SCOR, short-chain oxidoreductase; Usp, universal stress protein family; PBP, periplasmic binding protein; ABC, ATP-binding cassette
§2 Abbreviations of the protein names: FnBPA, fibronectin binding protein A; Ebh, extracellular matrix binding protein homologue; IspD, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
^¶ The reading frame is in relation to fliC and flag sequences
# Molecular mass in kilodaltons. The molecular mass of FliC_1-20 and FLAG-tag included when present in the gene product

ISSN: 1471-2180