Figure 5From: The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicansAdhesion analysis of the mp65Δ mutant. (A) Adhesion of the mp65Δ mutant to BEC. Representative fields randomly selected showing the interaction between yeast cells [wild type (wt), mp65Δ mutant (hom) and revertant (rev) strains] and BEC after 1 h of incubation at 37°C. The magnification bar corresponds to 100 μm. See the Methods section for more details. (B) Adhesion assay data. Histograms showing the adherence of the wild type (wt: black column), mp65Δ mutant (hom: grey column) and revertant (rev: white column) strains to BEC. The bars indicate the standard errors. Significant differences from wild type adhesion (P < 0.05) are indicated by asterisks. (C) Adhesion of the mp65Δ mutant to Caco-2 cell monolayers. Recovery of Candida cells [wild type (wt: black column), mp65Δ mutant (hom: grey column) and revertant (rev: white column) strains] at different time points (30 and 60 min) of incubation with Caco-2 cells. Adherent cells recovered after thorough washing out of the microplate (Panel 1). Non-adherent cells recovered from the supernatant (Panel 2). The results are the mean of 3 independent experiments. The bars indicate the standard deviations.Back to article page