From: Resistance to organic hydroperoxides requires ohr and ohrR genes in Sinorhizobium meliloti
Strains | genotype | origin |
---|---|---|
E. coli | ||
DH5α | endA-1 hsdR-17 supE-44 thi-1 recA-1 gyrA relA-1 Δ(lacZYA-argG)U169 deoR | [57] |
MT616 | MM294 pRK600 CmR | [58] |
BL21(DE3) | F- dcm ompT hsd gal/λ(DE3) | [59] |
Sinorhizobium meliloti | ||
Rm1021 | SU47 SmR | [60] |
R6.48 | Rm1021,ohrR::GmR | This study |
R7.15 | Rm1021,ΔohrR ohr::GmR | This study |
R7.16 | Rm1021,ohr+ ohrR+ ohr::lacZ ohrR::uidA | This study |
R8.39 | Rm1021,ohr::GmR | This study |
Plasmids | ||
pGEMT | pUC derivative cloning vector, AmpR | Promega |
pGEMTeasy | pUC derivative cloning vector, AmpR | Promega |
pET22b+ | expression vector, AmpR | Novagen |
pK18mobsacB | mobilisable pUC derivative, sacB NeoR | [51] |
pBBR1-MCS2 | broad host range replicating mobilisable vector, NeoR | [61] |
pBBR1-MCS5 | broad host range replicating mobilisable vector, GmR | [61] |
pTH1505 | GmR, gfp, lacZ, uidA, rfp fusion vector | [54] |
p34SGm | ori ColEI AmpR GmRcassette | [52] |
pD3001 | pK18mobsacB (XbaI-PstI)/ohrR downstream region (XbaI-NsiI) | this study |
pD3083 | pGEMTeasy/ ohrR upstream region | This study |
pD4116 | pK18mobsacB ΔohrR | This study |
pD4244 | pK18mobsacB ΔohrR::GmR | This study |
pD5333 | pK18mobsacBΔohrR ohr::GmR | This study |
pD5455 | pTH1505 ohr::lacZ, ohrR::uidA | This study |
pD8657 | pK18mobsacB ohr::GmR | This study |
pBBohr | pBBRI-MCS2 ohr+ | This study |
pBBohrR | pBBRI-MCS2 ohrR+ | This study |
pE1541 | pBBRI-MCS2 ohr::lacZ | This study |
pETohrR | pET22b+ ohr+ | This study |