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Table 5 Bacterial Strains, Plasmids and Phage

From: Identification and characterization of the intracellular poly-3-hydroxybutyrate depolymerase enzyme PhaZ of Sinorhizobium meliloti

Strain or Plasmid

Relevant Characteristics

Reference

S. meliloti

  

Rm5000

SU47 rif5

[22]

Rm1021

SU47 str-21, SmR

[50]

Rm11105

Rm1021 phaC 1::Tn5

[23]

Rm11107

Rm1021 bdhA 1::Tn5

[23]

Rm11144

Rm1021 phaC 1::Tn5 -233

[23]

Rm11347

Rm1021 phaB::ΩSmSp

[24]

Rm11417

Rm5000 phaZ::ΩSmSp

This work

Rm11430

Rm1021 phaZ::ΩSmSp

This work

Rm8369

Rm8002 exoF 369::TnphoA

[27]

E. coli

  

DH5α

F' endA1 hsdR17 (r K m+) supE44 thi-1 recA1 gyrA NalRrelA1 Δ(lacIZYA-argF) U169 deoR (ϕ 80dlac Δ(lacZ)M15)

[51]

MT607

pro-82 thi-1 hsdR17 supE44 recA56

[52]

MT616

MT607 pRK600

[52]

Plasmids

  

pK19mobsacB

Suicide vector KmR

[53]

pGEMTEasy

Cloning vector for PCR-generated DNA fragments, AmpR

Promega

pAZ101

pGEMTeasy carrying 835 bp fragment of SMc02770

This work

pAZ102

pAZ101 phaZ::OSmSp

This work

pAZ103

pK19mobsacB phaZ::ΩSmSp

This work

pRK7813

RK2 derivative carrying pUC9 polylinker. TcR

[54]

pMA157

pRK7813 SMc02770

This work

pD82

pLAFR1 cosmid clone from Rm1021 library carrying exoF and neighbouring genes

[26]

pD82exoF::TnphoA

pD82 exoF::TnphoA

This work

Phage

  

Ï• M12

S. meliloti transducing phage

[22]