From: Oligonucleotide microarray for the identification of potential mycotoxigenic fungi
Probe | Probe sequence (5' → 3')a | Probe specificityb | PCR annealing temperature (°C) for amplification | Reference (NCBI accession number) |
---|---|---|---|---|
Internal Transcribed regions | ||||
AaF AaR | GACCGCT TT C GT GGTATGCA | Alternaria alternata | 56 | This study [GenBank:FJ864712] |
AR1 | ATCTGCTGCACAGTTGGCT | Aspergillus carbonarius | 56 | This study [GenBank:FJ864707] |
AcarF AcarR | TGGCA CC AT TCGTCCTAC CCCGAGGCAGAGATG | Aspergillus carbonarius | 55 | This study [Genbank:FJ864707] |
AClF | ATTCGGAAACCUGCTCAGTACG | Aspergillus clavatus | 58 | This study [Genbank:EU515153, EF669942] |
AclaF AclaR | GCCGCCGTCTTCGGA CGTGTTGT AC AAC GTTTA | Aspergillus clavatus | 57 | This study [Genbank:EU078633] |
ApaF ApaR | GTGTAC GA GT TCCTAGCG GCCCGGGCTGACG | Aspergillus parasiticus | 55 | This study [GenBank:FJ864709] |
AVER | CCAACG CAG TTA CTTCA | Aspergillus versicolor | 56 | This study [GenBank:FJ864703] |
ANIG | ACGTTATCCAACCAT | Aspergillus niger | 55 | This study [GenBank:FJ864708] |
AnigF AnigR | ATTCGCCGGAGACCCCAACA TGTTGAAAGTTTTAACTGATTGCATT | Aspergillus niger | 55 | This study [GenBank:FJ864708] |
EurAF EurAR | TGGCGGCACCATGTC TGGTTAAAAGATTGGTTGCGA | Eurotium amstelodami | 58 | This study [GenBank:FJ864711] |
SL24F SL24R | CGGAAGGATCATTACTGAGTG GCCCGCCGAAGCAAC | Penicillium spp., Aspergillus spp. | 58 | This study [Genbank:AM270353, AM270995, DQ469292, DQ249211] |
IT59 ITS60 | CGTGTTTATTTACCT ACAGAGCGGTGACA | Penicillium spp. | 58 | This study [EU7975707.1] |
PenCorF PenCorR | GTCCAAACCCTCCCACCCA GTCAGACTTGCAATCTTCAGACTGT | Penicillium corylophilum | 55 | This study [FJ864704] |
PenExF PenExR | TTACCGAGTGAGGCCGT GCCA GCCT GAC AGCTACG | Penicllium expansum | 58 | This study [Genbank:FJ861424] |
PenFeF PenFeR | CTGAGTGCGGGCCCTCT CGCCGAAGCAACACTGTAAG | Penicillium fellutanum | 55 | This study [Genbank:EF200082] |
PenIsF PenIsR | CGAGTGCGGGTTCGACA GGCAACGCGGTAACGGTAG | Penicilliun islandicum | 57 | This study [Genbank:AF455543] |
PenItF PenItR | CTCCCACCCGTGTTTATTTATCA TCACTCAGACGACAATCTTCAGG | Penicillium italicum | 57 | This study [Genbank:DQ991463] |
ITSF ITSR | CAACTCCAAACCCCTGTGA GCGACGATTACCAGTAACGA | Fusarium spp. | 58 | [34] |
NSA3 NSI1 | AAACCCTGTCGTGCTGGGGATA GATTGAATGGCTCGGTGAGG | Internal Transcribed Region | 60 | [35] |
NLB4 NLC2 | GGATTCTCACCCTCTATGAC GAGCTGCATTCCCAAACAACTC | Internal Transcribed Region | 58 | [35] |
4F 4R | CAAACGTCGGGTCAGAAGAAGCGAC AGGAACCGTCCCCGCCGACGTTTG | Styenocarpella maydis | 57 | [36] |
Elongation Factor 1-alpha genes | ||||
EF526F EF1567R | GTCCTGATAGGHCACGT ACGGTCTATACCACCRATCTT | Fusarium spp. | 57 | This study [Genbank:FJ864705] |
FaF FaR | CAAGCATTGTCGCCACTCTC GTTTGGCTCTACCGGGACTG | Fusarium avenaceum | 60 | [37] |
FAC-F FAC-R | GGGATATCGGGCCTCA GGGATATCGGCAAGATCG | Fusarium acuminatum | 57 | [38] |
CroAF CroAR | CTCAGTGTCCACCGCGTTGCGTA CTCAGTGTT CCG AATCT AATAGTCC | Fusarium crookwellense | 60 | [39] |
198F2 198R1 | GACAGCAAGATTGACCTTTTGG GACATACTCTACAAGTGCCAA | Fusarium equiseti | 58 | [40] |
Fg16NF Fg16NR | ACAGATGACAAGATTCAGGCACA TTCTTTGACATCTGTTCAACCCA | Fusarium graminearum | 57 | [41] |
FGDF FGDR | ACATACCACTTGTTGCCTCG CGCCAATCAATTTGAGGAACG | Fusarium graminearum | 55 | This study [Genbank:XM-388987] |
FspoF1 LanSpoR1 | CGCACAT ACC CT AACTCATC TACAAGAAGAGCGTGGCGATAT | Fusarium sporotrichioides | 58 | This study [Genbank:FJ864706] |
61-2F 61-2R | GGCCACTA AT GAC GCGAAAG GTCAGACCAGAGCAATGGGC | Fusarium subglutinans | 60 | [40] |
VER1 VER2 | CTTCCTGCGATGTTTCTCC AATTGGCCATGGTATTATATATCTA | Fusarium verticillioides | 56 | [42] |
53-6F 53-6R | TTACGAGGCGGCGATGGGT GGCCGTTTACCTGGCTTCTT | Fusarium verticillioides | 60 | [40] |
Mycotoxin genes | ||||
NORTAQ-1 | GTCCAAGCAACAGGCCAAGT | Aflatoxin | 56 | [43] |
NORTAQ-2 | TCGTGCATGTTGGTGATGGT | Aflatoxin | 56 | [43] |
NORPROBE | TGTCTTGATCGGCGCCCG | Aflatoxin | 56 | [43] |
IDH 2076F IDH 2667R | GCCCATGTGCTCATTACAG TGGGACAATTCCTGAACATGC | Iso-epoxy dehydrogenase | 58 | [44] |
IDH 2195F IDH 2793R | CAATGTGTCGTATGTGCCC ACCTTCAGTCGCTGTTCCTC | Iso-epoxy dehydrogenase | 59 | [44] |
IDH1 IDH2 | CAATGTGTCGTACTGTGCCC ACCTTCAGTCGCTGTTCCTC | Iso-epoxy dehydrogenase | 59 | [45] |
Tri7F2 Tri7DON | GTGCGTGGCAATATCTTCTTAGTTA GTGTAATATTGTGCTAATATTGTGC | Deoxynivalenol | 58 | [46] |
Tri13F Tri13DON | CATCATGAGACTTGTKCRAGTTTGG GCTAGATCGATTGTTGCATTGAG | Deoxynivalenol | 58 | [46] |
Fum5F Fum5R | GTCGAGTTGTTGACCACTGC CGTATCGTCAGCATGATGTAGC | Fumonisin | 60 | [34] |
Tri7F Tri7NIV | TGCTGTGGCAATATCTTCTTCTA GGTTCAAGTAACGTTCGACAATA | Nivalenol | 58 | [46] |
Tri13NIVF Tri13R | CCAAATCCGAAAACCGCA TTGAAAGCTCCAATGTCCGTG | Nivalenol | 57 | [46] |
Tri5F Tri5R | AGCGACTACAGGCTTCCCTC AATTCTCCATCTGACCATCCAG | Trichothecenes | 58 | [47] |
TOX5F TOX5R | GCTGCTCATCACTTTGCTCA CTGATCTGGTCACGCTCATC | Trichothecenes | 59 | [48] |
Positive controls | ||||
ITS3 | GCATCGATGAAGAACGCAGC | Positive hybridization control | 55 | White et al, 1990 |
ITS1 ITS4 | TCCGTAGGTGAACCTGCG TCCTCCGCTTATTGATATGC | Positive hybridization control | 55 | White et al, 1990 |