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Table 1 Probe sequences and names of species- and toxin- specific genes for different fungal isolates

From: Oligonucleotide microarray for the identification of potential mycotoxigenic fungi

Probe

Probe sequence (5' → 3')a

Probe specificityb

PCR annealing temperature (°C) for amplification

Reference (NCBI accession number)

Internal Transcribed regions

AaF

AaR

GACCGCT TT C GT GGTATGCA

Alternaria alternata

56

This study [GenBank:FJ864712]

AR1

ATCTGCTGCACAGTTGGCT

Aspergillus carbonarius

56

This study [GenBank:FJ864707]

AcarF

AcarR

TGGCA CC AT TCGTCCTAC

CCCGAGGCAGAGATG

Aspergillus carbonarius

55

This study [Genbank:FJ864707]

AClF

ATTCGGAAACCUGCTCAGTACG

Aspergillus clavatus

58

This study

[Genbank:EU515153, EF669942]

AclaF

AclaR

GCCGCCGTCTTCGGA

CGTGTTGT AC AAC GTTTA

Aspergillus clavatus

57

This study

[Genbank:EU078633]

ApaF

ApaR

GTGTAC GA GT TCCTAGCG

GCCCGGGCTGACG

Aspergillus parasiticus

55

This study [GenBank:FJ864709]

AVER

CCAACG CAG TTA CTTCA

Aspergillus versicolor

56

This study [GenBank:FJ864703]

ANIG

ACGTTATCCAACCAT

Aspergillus niger

55

This study

[GenBank:FJ864708]

AnigF

AnigR

ATTCGCCGGAGACCCCAACA

TGTTGAAAGTTTTAACTGATTGCATT

Aspergillus niger

55

This study [GenBank:FJ864708]

EurAF

EurAR

TGGCGGCACCATGTC

TGGTTAAAAGATTGGTTGCGA

Eurotium amstelodami

58

This study [GenBank:FJ864711]

SL24F

SL24R

CGGAAGGATCATTACTGAGTG

GCCCGCCGAAGCAAC

Penicillium spp., Aspergillus spp.

58

This study

[Genbank:AM270353, AM270995, DQ469292, DQ249211]

IT59

ITS60

CGTGTTTATTTACCT

ACAGAGCGGTGACA

Penicillium spp.

58

This study

[EU7975707.1]

PenCorF

PenCorR

GTCCAAACCCTCCCACCCA

GTCAGACTTGCAATCTTCAGACTGT

Penicillium corylophilum

55

This study

[FJ864704]

PenExF

PenExR

TTACCGAGTGAGGCCGT

GCCA GCCT GAC AGCTACG

Penicllium expansum

58

This study

[Genbank:FJ861424]

PenFeF

PenFeR

CTGAGTGCGGGCCCTCT

CGCCGAAGCAACACTGTAAG

Penicillium fellutanum

55

This study

[Genbank:EF200082]

PenIsF

PenIsR

CGAGTGCGGGTTCGACA

GGCAACGCGGTAACGGTAG

Penicilliun islandicum

57

This study

[Genbank:AF455543]

PenItF

PenItR

CTCCCACCCGTGTTTATTTATCA

TCACTCAGACGACAATCTTCAGG

Penicillium italicum

57

This study

[Genbank:DQ991463]

ITSF

ITSR

CAACTCCAAACCCCTGTGA

GCGACGATTACCAGTAACGA

Fusarium spp.

58

[34]

NSA3

NSI1

AAACCCTGTCGTGCTGGGGATA

GATTGAATGGCTCGGTGAGG

Internal Transcribed Region

60

[35]

NLB4

NLC2

GGATTCTCACCCTCTATGAC

GAGCTGCATTCCCAAACAACTC

Internal Transcribed Region

58

[35]

4F

4R

CAAACGTCGGGTCAGAAGAAGCGAC

AGGAACCGTCCCCGCCGACGTTTG

Styenocarpella maydis

57

[36]

Elongation Factor 1-alpha genes

EF526F

EF1567R

GTCCTGATAGGHCACGT

ACGGTCTATACCACCRATCTT

Fusarium spp.

57

This study

[Genbank:FJ864705]

FaF

FaR

CAAGCATTGTCGCCACTCTC

GTTTGGCTCTACCGGGACTG

Fusarium avenaceum

60

[37]

FAC-F

FAC-R

GGGATATCGGGCCTCA

GGGATATCGGCAAGATCG

Fusarium acuminatum

57

[38]

CroAF

CroAR

CTCAGTGTCCACCGCGTTGCGTA

CTCAGTGTT CCG AATCT AATAGTCC

Fusarium crookwellense

60

[39]

198F2

198R1

GACAGCAAGATTGACCTTTTGG

GACATACTCTACAAGTGCCAA

Fusarium equiseti

58

[40]

Fg16NF

Fg16NR

ACAGATGACAAGATTCAGGCACA

TTCTTTGACATCTGTTCAACCCA

Fusarium graminearum

57

[41]

FGDF

FGDR

ACATACCACTTGTTGCCTCG

CGCCAATCAATTTGAGGAACG

Fusarium graminearum

55

This study

[Genbank:XM-388987]

FspoF1

LanSpoR1

CGCACAT ACC CT AACTCATC

TACAAGAAGAGCGTGGCGATAT

Fusarium sporotrichioides

58

This study

[Genbank:FJ864706]

61-2F

61-2R

GGCCACTA AT GAC GCGAAAG

GTCAGACCAGAGCAATGGGC

Fusarium subglutinans

60

[40]

VER1

VER2

CTTCCTGCGATGTTTCTCC

AATTGGCCATGGTATTATATATCTA

Fusarium verticillioides

56

[42]

53-6F

53-6R

TTACGAGGCGGCGATGGGT

GGCCGTTTACCTGGCTTCTT

Fusarium verticillioides

60

[40]

Mycotoxin genes

NORTAQ-1

GTCCAAGCAACAGGCCAAGT

Aflatoxin

56

[43]

NORTAQ-2

TCGTGCATGTTGGTGATGGT

Aflatoxin

56

[43]

NORPROBE

TGTCTTGATCGGCGCCCG

Aflatoxin

56

[43]

IDH 2076F

IDH 2667R

GCCCATGTGCTCATTACAG

TGGGACAATTCCTGAACATGC

Iso-epoxy dehydrogenase

58

[44]

IDH 2195F

IDH 2793R

CAATGTGTCGTATGTGCCC

ACCTTCAGTCGCTGTTCCTC

Iso-epoxy dehydrogenase

59

[44]

IDH1

IDH2

CAATGTGTCGTACTGTGCCC

ACCTTCAGTCGCTGTTCCTC

Iso-epoxy dehydrogenase

59

[45]

Tri7F2

Tri7DON

GTGCGTGGCAATATCTTCTTAGTTA

GTGTAATATTGTGCTAATATTGTGC

Deoxynivalenol

58

[46]

Tri13F

Tri13DON

CATCATGAGACTTGTKCRAGTTTGG

GCTAGATCGATTGTTGCATTGAG

Deoxynivalenol

58

[46]

Fum5F

Fum5R

GTCGAGTTGTTGACCACTGC

CGTATCGTCAGCATGATGTAGC

Fumonisin

60

[34]

Tri7F

Tri7NIV

TGCTGTGGCAATATCTTCTTCTA

GGTTCAAGTAACGTTCGACAATA

Nivalenol

58

[46]

Tri13NIVF

Tri13R

CCAAATCCGAAAACCGCA

TTGAAAGCTCCAATGTCCGTG

Nivalenol

57

[46]

Tri5F

Tri5R

AGCGACTACAGGCTTCCCTC

AATTCTCCATCTGACCATCCAG

Trichothecenes

58

[47]

TOX5F

TOX5R

GCTGCTCATCACTTTGCTCA

CTGATCTGGTCACGCTCATC

Trichothecenes

59

[48]

Positive controls

ITS3

GCATCGATGAAGAACGCAGC

Positive hybridization control

55

White et al, 1990

ITS1

ITS4

TCCGTAGGTGAACCTGCG

TCCTCCGCTTATTGATATGC

Positive hybridization control

55

White et al, 1990

  1. a Locked nucleic acids (LNAs) that were used to increase the specificity of a probe are in bold and underlined.
  2. b Fungal names should be treated as sensu stricto.