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Figure 3 | BMC Microbiology

Figure 3

From: Genetic analysis of the Staphylococcus epidermidis Macromolecular Synthesis Operon: Serp1129 is an ATP binding protein and sigA transcription is regulated by both σA- and σB-dependent promoters

Figure 3

Northern blot analysis of the S. epidermidis MMSO using a sigA and dnaG DNA probe. The number above each lane in panels A (hybridized with a sigA probe) and B (hybridized with a dnaG probe) represents the time in hours of growth before each RNA sample was processed. A picture of the ethidium bromide stained gel is shown beneath each blot to serve as a loading control and verify RNA integrity. Arrows in panels A and B denote transcripts A, C through F as discussed in text. Panel C: Schematic depiction of the S. epidermidis MMSO. Small arrows above and below the schematic represent primer sets used in RT-PCR reactions and other cloning experiments. Arrows below the schematic correspond to transcripts A, B, C, and D as discussed in text.

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